Photoaptamer arrays applied to multiplexed proteomic analysis
| Autor: | Glenn Foulds, Daniel J. Schneider, Brian Collins, Michele Nelson Hurst, Helen H. Petach, Larry Gold, Sheri K. Wilcox, Jim Heil, Sheela Waugh, Brian Hicke, Allison Weiss, Joseph S Heilig, Jody Davis, Chad Greef, Dominic Zichi, Michael Patrick Coleman, Barry Vant-Hull, Chris Bock, Gregory M. Husar, Rachel Ostroff, Darcey Miller |
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| Rok vydání: | 2004 |
| Předmět: |
Proteomics
Vascular Endothelial Growth Factor A Analyte Light Aptamer Analytical chemistry Oligonucleotides Protein Array Analysis Biochemistry Antibodies Humans Neoplasm Metastasis Molecular Biology Detection limit Interleukin-16 Chromatography Tissue Inhibitor of Metalloproteinase-1 Dose-Response Relationship Drug Chemistry Proteins DNA Hydrogen-Ion Concentration Fluorescence Endostatins Kinetics Cross-Linking Reagents Reagent Colonic Neoplasms Protein microarray Fibroblast Growth Factor 2 Lod Score |
| Zdroj: | Proteomics. 4(3) |
| ISSN: | 1615-9853 |
| Popis: | Multiplexed photoaptamer-based arrays that allow for the simultaneous measurement of multiple proteins of interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, the arrays can be vigorously washed to remove background proteins, providing the potential for superior signal-to-noise ratios and lower limits of quantification in biological matrices. Data are presented here for a 17-plex photoaptamer array exhibiting limits of detection below 10 fM for several analytes including interleukin-16, vascular endothelial growth factor, and endostatin and able to measure proteins in 10% serum samples. The assays are simple, scalable, and reproducible. Affinity of the capture reagent is shown to be directly correlated to the limit of detection for the analyte on the array. |
| Databáze: | OpenAIRE |
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