Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6
| Autor: | Tomonobu Koizumi, Mitsutoshi Sugano, Masaki Yamaguchi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Blood Platelets
Medicine (General) Erythrocytes Biochemistry Specimen Handling Pre-Clinical Research Report storage Leukocyte Count Plasma R5-920 White blood cell medicine Leukocytes Humans Interleukin 6 Sample handling Plasma samples biology business.industry Interleukin-6 Biochemistry (medical) Interleukin-8 Cell Biology General Medicine medicine.anatomical_structure Healthy individuals Immunology biology.protein Biomarker (medicine) biomarker ultra-low level white blood cell business |
| Zdroj: | The Journal of International Medical Research Journal of International Medical Research, Vol 49 (2021) |
| ISSN: | 1473-2300 |
| Popis: | Objective To quantitate plasma interleukin-6 (IL-6) levels in healthy individuals and to clarify how these levels are affected by blood sample handling procedures during short-term storage. Methods Ethylenediaminetetraacetic acid (EDTA)-treated plasma samples were simultaneously collected from 14 healthy individuals and stored on ice prior to analysis of the IL-6 levels. White blood cells (WBCs), red blood cells, and platelets were counted immediately after blood collection. IL-6 levels were analyzed every 30 minutes using a commercial electrochemiluminescence immunoassay. Results Correlation coefficients between plasma IL-6 levels and WBC counts ranged between 0.605 and 0.554, higher than those for other cell types. The lowest IL-6 value in healthy individuals was estimated at 0.04 pg/mL and the mean values remained under 2 pg/mL over time. Conclusion Analysis of IL-6 levels in EDTA-treated plasma samples centrifuged within 1 hour and stored on ice can be performed within 90 minutes of short-term storage if the analytical method has a sensitivity in the range of 10 fg/mL. |
| Databáze: | OpenAIRE |
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