Silole nanocrystals as novel biolabels
| Autor: | King-keung Sin, Ben Zhong Tang, Yongqiang Dong, Matthias Haeussler, Matthias Seydack, Cangel Pui-yee Chan, Dieter Trau, Reinhard Renneberg, Wing Cheung Mak |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Immunoassay
chemistry.chemical_classification medicine.diagnostic_test Biomolecule Immunology Pilot Projects Nanotechnology Fluorescence Nanocrystalline material Polyelectrolyte Highly sensitive chemistry Nanocrystal Spectrophotometry medicine Immunology and Allergy Crystallization Agrégation Fluorescent Dyes |
| Zdroj: | Journal of Immunological Methods. 295:111-118 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/j.jim.2004.09.016 |
| Popis: | A novel class of biofunctional silole nanocrystals with the potential to create highly sensitive immunoassay was firstly demonstrated. Biolabels were constructed by encapsulating nanocrystalline hexaphenylsilole [Ph2Si(CPh)4HPS] within ultrathin polyelectrolyte layers via the layer-by-layer (LbL) technique that provided an "interface" for the attachment of antibodies. A high ratio of fluorescent dyes to biomolecules (F/P ratio; 2.4 x 10(3)) was achieved without self-quenching problem. The aggregation-induced emission (AIE) feature offered silole biolabels the sensitivity 40- to 140-fold higher than that of a start-of-the-art immunoassay using directly fluorescent-labeled antibodies. |
| Databáze: | OpenAIRE |
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