Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina Phaseolina in Soybean (Glycine Max L.)
| Autor: | Humaira Yasmin, Zhide Geng, Asia Nosheen, Noshin Ilyas, Xiangkuo Gao, Seemab Anjum, Muhammad Nadeem Hassan, Muhammad Sajjad, Rabia Naz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
PGPB salicylic acid lcsh:TJ807-830 Geography Planning and Development charcoal rot lcsh:Renewable energy sources Management Monitoring Policy and Law 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Proline biocontrol lcsh:Environmental sciences 030304 developmental biology lcsh:GE1-350 0303 health sciences biology Renewable Energy Sustainability and the Environment lcsh:Environmental effects of industries and plants Jasmonic acid fungi Bacillus clausii jasmonic acid food and beverages biology.organism_classification Macrophomina phaseolina Pseudomonas putida Fungicide Horticulture lcsh:TD194-195 chemistry Shoot Salicylic acid 010606 plant biology & botany |
| Zdroj: | Sustainability Volume 12 Issue 17 Sustainability, Vol 12, Iss 6856, p 6856 (2020) |
| ISSN: | 2071-1050 |
| DOI: | 10.3390/su12176856 |
| Popis: | Controlling agricultural pests using suitable biocontrol agents has been considered the best strategy for sustainable agriculture. Charcoal rot caused by a necrotrophic fungus Macrophomina phaseolina is responsible for a 30&ndash 50% annual reduction in soybean yield worldwide. Little is known about the role of Bacillus clausii in reducing charcoal rot disease severity in the soybean crop. In this study, we investigated plant growth promoting and antagonistic potential of Pseudomonas putida (MT604992) and Bacillus clausii (MT604989) against charcoal rot disease incidence in soybean. Among twenty bacteria isolated from soil and water samples of two different hot springs of Gilgit-Baltistan, Pakistan, 80% were siderophore positive 65% were hydrogen cyanide (HCN) positive 55%, 30%, and 75% were phosphate, potassium, and zinc solubilizers, respectively. Based on higher antagonistic activities and plant growth promoting traits five strains were selected for in vitro screening. Out of all tested strains, Pseudomonas putida and Bacillus clausii showed a significant increase in germination, growth, and disease suppression in soybean. These strains produced a pronounced increase in relative water content, photosynthetic pigments, membrane stability, proline, antioxidant enzymes status, phytohormones content (Salicylic acid, and Jasmonic acid), and disease suppression in comparison to control plants. Bacillus clausii mitigated the disease by 97% with a marked increase in the proline content (73% and 89%), superoxide dismutase (356% and 208%), peroxidase (439% and 138.6%), catalase (255.8% and 80.8%), and ascorbate peroxidase (228% and 90%) activities in shoots and roots, respectively. Infected plants showed an increase in salicylic acid and jasmonic acid content which was further increased with the application of the selected strains to increase resistance against pathogens. To our knowledge, this is the first study showing a rise in salicylic acid and jasmonic acid in Macrophomina phaseolina infected plants. These two strains are suggested as a cost-effective, eco-friendly, and sustainable alternative to chemical fungicides. However, there is a need to explore the field testing and molecular mechanisms leading to disease suppression by these strains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|