Serum-induced cytosolic calcium movements and mitogenesis in cultured preosseous chondrocytes
| Autor: | Micaela Grandolfo, Paola D'Andrea, Benedetto de Bernard, Franco Vittur |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Swine Cellular differentiation chemistry.chemical_element In Vitro Techniques Biology Calcium Chondrocyte Calcium in biology Cytosol Internal medicine medicine Animals Cells Cultured Fetus DNA synthesis Cell growth DNA Cell Biology Fetal Blood Cartilage medicine.anatomical_structure Endocrinology chemistry Cattle Fura-2 Cell Division Intracellular |
| Zdroj: | Experimental Cell Research. 191:22-26 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(90)90030-e |
| Popis: | The differentiation of preosseous chondrocytes begins with the proliferation of resting cells and results in the expression of the hypertrophic phenotype. The effect of fetal calf serum on chondrocyte mitogenesis and intracellular Ca2+ concentration was studied in resting and hypertrophic cells in primary culture. Resting chondrocytes respond to the growth stimulus with immediate release of Ca2+ from intracellular stores and with opening of the plasma membrane Ca2+ channels. These events may be related to the elevated [3H]thymidine incorporation observed after serum exposure. In contrast, in hypertrophic chondrocytes the lower rate of DNA synthesis seems to be coupled with a lower activity of the Ca2+ signaling mechanism and, probably, with reduced intracellular calcium stores. It is proposed that expression of the Ca2+ signaling mechanism may be modulated during the differentiation of preosseous chondrocytes. |
| Databáze: | OpenAIRE |
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