Understanding host-microbiota interactions in the commercial piglet around weaning

Autor: Saladrigas-García, M., D’Angelo, M., Ko, H.L., Nolis, P., Ramayo-Caldas, Y., Folch, J.M., Llonch, P., Solà-Oriol, D., Pérez, J.F., Martín-Orúe, S.M.
Přispěvatelé: Ministerio de Economía, Industria y Competitividad (España), Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Universidad Autónoma de Barcelona, Generalitat de Catalunya, Producció Animal, Genètica i Millora Animal
Rok vydání: 2021
Předmět:
Zdroj: Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
Digital.CSIC. Repositorio Institucional del CSIC
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Scientific Reports
IRTA Pubpro. Open Digital Archive
Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
ISSN: 2045-2322
DOI: 10.1038/s41598-021-02754-6
Popis: Weaning is a critical period in the life of pigs with repercussions on their health and welfare and on the economy of the swine industry. This study aimed to assess the efect of the commercial early weaning on gut microbiota, intestinal gene expression and serum metabolomic response via an integratedomic approach combining 16S rRNA gene sequencing, the OpenArray gene expression technology and 1 H-NMR spectroscopy. Fourteen piglets from diferent litters were sampled for blood, jejunum tissue and caecal content two days before (− 2d), and three days after (+3d) weaning. A clearly diferential ordination of caecal microbiota was observed. Higher abundances of Roseburia, Ruminococcus, Coprococcus, Dorea and Lachnospira genera in weaned piglets compared to prior to weaning showed the quick microbial changes of the piglets’ gut microbiota. Downregulation of OCLN, CLDN4, MUC2, MUC13, SLC15A1 and SLC13A1 genes, also evidenced the negative impact of weaning on gut barrier and digestive functions. Metabolomic approach pinpointed signifcant decreases in choline, LDL, triglycerides, fatty acids, alanine and isoleucine and increases in 3-hydroxybutyrate after weaning. Moreover, the correlation between microbiota and metabolome datasets revealed the existence of metabolic clusters interrelated to diferent bacterial clusters. Our results demonstrate the impact of weaning stress on the piglet and give insights regarding the associations between gut microbiota and the animal gene activity and metabolic response.
We would like to acknowledge the funding from the Ministry of Economy, Industry and Competitiveness (MINECO) of Spain for granting the project AGL2016-75463-R within the framework of Proyectos I+D+I Convocatoria RETOS 2016 and the State Plan for Scientific and Technical Research and Innovation. MS received an FPI grant from the Spanish Ministry of Science and Innovation (grant number BES-2017-080018). MD received support from Opening Sphere UAB-CEI to Postdoctoral Fellows (project H2020-MSCA-COFUND-2014). HLK received the University, Research Center and Hospital Foundation Grants for the Contracting of New Research Staff (FI) from Agency for Management of University and Research Grants (AGAUR) of the Catalan Government. PL received support from the Tecniospring program (TECSPR15-1-0040) of ACCIÓ funded by the Catalan Government and the Marie Curie COFUND Fellowship Program within the 7th European Community Framework. YRC is recipient of a Ramon y Cajal post-doctoral fellowship (RYC2019-027244-I) from the Spanish Ministry of Science and Innovation.
Databáze: OpenAIRE
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