Identification of viral agents associated with diarrhea in young children during a winter season in Beijing, China
| Autor: | Lena Grillner, Annika Linde, Benita Zweygberg Wirgart, Chunyan Liu, Kunling Shen, Kari Johansen, Klas Jönsson, Annika Tiveljung Lindell |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Diarrhea
Rotavirus Male China viruses Reoviridae medicine.disease_cause Virus Article dNTP deoxynucleotide triphosphate Astrovirus Immunoenzyme Techniques Virus antigen fluids and secretions RT-PCR reverse transcription polymerase chain reaction Virology medicine Humans EM electron microscopy EIA enzyme immunoassay biology Reverse Transcriptase Polymerase Chain Reaction Adenoviruses Human Norovirus virus diseases Infant biology.organism_classification Infectious Diseases El Niño Virus Diseases Child Preschool Viruses RNA ribonucleic acid RNA Viral Female Enteric adenovirus Seasons medicine.symptom Mamastrovirus |
| Zdroj: | Journal of Clinical Virology |
| ISSN: | 1873-5967 1386-6532 |
| Popis: | Background Viral diarrhea remains a major cause of childhood morbidity and mortality worldwide. Although rotavirus was extensively studied in China, few comprehensive studies of all viral agents related to diarrhea in children have been conducted. Objectives Our study was performed to investigate the role of enteric viruses in acute diarrhea in our country and to evaluate methods that could be used in routine diagnostics. Study design One hundred stool samples were collected from children under 5 years of age seeking medical care for acute diarrhea during the winter season 2000/2001 in Beijing Children's Hospital. All specimens were initially screened microscopically for leucocytes/red blood cells. Samples with negative results were analyzed for virus presence using commercial EIAs and/or in-house RT-PCRs. Results At least one viral agent was found in 67% of the specimens. The frequency of rotavirus, astrovirus, norovirus and enteric adenovirus was 59%, 8%, 6% and 2%, respectively. Dual infections were found in 9.0% (6/67) of the positive samples. The results from rotavirus and astrovirus EIAs were concordant with those of rotavirus and astrovirus RT-PCRs. Conclusions Enteric viruses play an important role in pediatric diarrhea during the winter season in China. A combination of microscopic examination of stool samples with specific EIA assays to detect virus antigen in stool specimens may be suitable for routine diagnostics. |
| Databáze: | OpenAIRE |
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