Spatial control of lipid droplet proteins by the ERAD ubiquitin ligase Doa10
| Autor: | Gabriel Mora, Pedro Carvalho, Laura Buxó, Annamaria Ruggiano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligases Endoplasmic-reticulum-associated protein degradation Protein degradation Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Gene Expression Regulation Fungal Lipid droplet Inner membrane Membrane & Intracellular Transport Molecular Biology General Immunology and Microbiology biology General Neuroscience Endoplasmic reticulum Lipid Droplet Associated Proteins Articles Protein Biosynthesis & Quality Control ERAD Doa10 Lipid droplets Protein subcellular localization prediction 3. Good health Ubiquitin ligase Cell biology 030104 developmental biology Membrane protein Proteolysis biology.protein _ |
| Zdroj: | The EMBO Journal Recercat. Dipósit de la Recerca de Catalunya instname |
| ISSN: | 1460-2075 0261-4189 |
| Popis: | The endoplasmic reticulum (ER) plays a central role in the biogenesis of most membrane proteins. Among these are proteins localized to the surface of lipid droplets (LDs), fat storage organelles delimited by a phospholipid monolayer. The LD monolayer is often continuous with the membrane of the ER allowing certain membrane proteins to diffuse between the two organelles. In these connected organelles, how some proteins concentrate specifically at the surface of LDs is not known. Here, we show that the ERAD ubiquitin ligase Doa10 controls the levels of some LD proteins. Their degradation is dependent on the localization to the ER and appears independent of the folding state. Moreover, we show that by degrading the ER pool of these LD proteins, ERAD contributes to restrict their localization to LDs. The signals for LD targeting and Doa10‐mediated degradation overlap, indicating that these are competing events. This spatial control of protein localization is a novel function of ERAD that might contribute to generate functional diversity in a continuous membrane system. A. Ruggiano was supported by a “La Caixa” graduate fellowship; P. Carvalho is supported by CRG, an International Early Career Award from the HHMI, the EMBO Young Investigator Program, and grants from the Spanish MCCIN and ERC (FP7/2007‐2013 ERC grant agreement no. 309477 DROPFAT). We acknowledge support of the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013–2017”, SEV‐2012‐0208. |
| Databáze: | OpenAIRE |
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