Light Induced Changes in Protein Expression and Uniform Regulation of Transcription in the Thylakoid Lumen of Arabidopsis thaliana
| Autor: | Thomas Kieselbach, Wolfgang P. Schröder, Irene Granlund, Michael N. Hall |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Photosystem II
Chemical Biology/Protein Chemistry and Proteomics Light Proteome Transcription Genetic Arabidopsis lcsh:Medicine Plant Biology/Plant-Environment Interactions macromolecular substances Photosynthesis Genes Plant environment and public health Thylakoids Plant Biology/Plant Biochemistry and Physiology Transcription (biology) Biochemistry/Protein Chemistry Gene Expression Regulation Plant Gene expression polycyclic compounds Cluster Analysis Electrophoresis Gel Two-Dimensional lcsh:Science Photosystem Multidisciplinary biology Chemistry Arabidopsis Proteins lcsh:R food and beverages Darkness biology.organism_classification Molecular biology Adaptation Physiological Chloroplast Thylakoid Biophysics lcsh:Q lipids (amino acids peptides and proteins) Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 4, Iss 5, p e5649 (2009) |
| ISSN: | 1932-6203 |
| Popis: | In plants oxygenic photosynthesis is performed by large protein complexes found in the thylakoid membranes of chloroplasts. The soluble thylakoid lumen space is a narrow and compressed region within the thylakoid membrane which contains 80-200 proteins. Because the thylakoid lumen proteins are in close proximity to the protein complexes of photosynthesis, it is reasonable to assume that the lumen proteins are highly influenced by the presence of light. To identify light regulated proteins in the thylakoid lumen of Arabidopsis thaliana we developed a faster thylakoid preparation and combined this with difference gel electrophoresis (DIGE) of dark-adapted and light-adapted lumen proteomes. The DIGE experiments revealed that 19 lumen proteins exhibit increased relative protein levels after eight hour light exposure. Among the proteins showing increased abundance were the PsbP and PsbQ subunits of Photosystem II, major plastocyanin and several other proteins of known or unknown function. In addition, co-expression analysis of publicly available transcriptomic data showed that the co-regulation of lumen protein expression is not limited to light but rather that lumen protein genes exhibit a high uniformity of expression. The large proportion of thylakoid lumen proteins displaying increased abundance in light-adapted plants, taken together with the observed uniform regulation of transcription, implies that the majority of thylakoid lumen proteins have functions that are related to photosynthetic activity. This is the first time that an analysis of the differences in protein level during a normal day/night cycle has been performed and it shows that even a normal cycle of light significantly influences the thylakoid lumen proteome. In this study we also show for the first time, using co-expression analysis, that the prevalent lumenal chloroplast proteins are very similarly regulated at the level of transcription. |
| Databáze: | OpenAIRE |
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