Flow‐Evoked Vasodilation Is Blunted in Penile Arteries from Zucker Diabetic Fatty Rats
| Autor: | Jørgen Bjerggaard Jensen, Allan Flyvbjerg, Olav L. Schjørring, Attila Kun, Ulf Simonsen, Hans Jørgen Kirkeby |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Urology Endocrinology Diabetes and Metabolism Indomethacin Vasodilation Arginine Nitric oxide Potassium Channels Calcium-Activated chemistry.chemical_compound Endocrinology Diabetes mellitus Internal medicine medicine Animals Cyclooxygenase Inhibitors biology business.industry Myography Arteries Iberiotoxin medicine.disease Rats Rats Zucker Endothelial stem cell Psychiatry and Mental health Reproductive Medicine chemistry biology.protein Cyclooxygenase Nitric Oxide Synthase Peptides business Acetylcholine Penis Myograph medicine.drug |
| Zdroj: | Schjørring, O, Kun, A, Flyvbjerg, A, Kirkeby, H J, Jensen, J B & Simonsen, U 2012, ' Flow-Evoked Vasodilation Is Blunted in Penile Arteries from Zucker Diabetic Fatty Rats ', Journal of Sexual Medicine, pp. 1789-1800 . https://doi.org/10.1111/j.1743-6109.2012.02743.x |
| ISSN: | 1743-6095 |
| DOI: | 10.1111/j.1743-6109.2012.02743.x |
| Popis: | Introduction Endothelium‐derived relaxing factors such as nitric oxide (NO), prostanoids, and endothelium‐derived hyperpolarizing factor (EDHF) are thought to play an important role in vasodilation of penile arteries. Aim The present study investigated the mechanisms involved in flow‐ and acetylcholine‐induced vasodilation in penile arteries, and whether acetylcholine‐ and flow‐mediated vasodilation is altered in Zucker diabetic fatty (ZDF) rats, a model of type 2 diabetes. Moreover, it was addressed whether enhanced myogenic tone may explain impaired flow‐evoked vasodilation in arteries from ZDF rats. Methods Penile dorsal arteries obtained from lean control and ZDF rats were suspended in a pressure myograph, and flow‐ and acetylcholine‐evoked vasodilation was measured as changes in arterial diameter. Main Outcome Measure Changes in penile arterial diameter. Results Incubation with an inhibitor of NO synthase, asymmetric dimethyl‐L‐arginine (ADMA), and of cyclooxygenase, indomethacin, reduced acetylcholine but not flow‐evoked vasodilation in penile arteries, while both responses were abolished by endothelial cell removal. Iberiotoxin, a blocker of large‐conductance calcium‐activated K + (BK Ca ) channels, inhibited flow‐evoked vasodilation. Flow‐evoked vasodilation was reduced in arteries from ZDF rats in the absence, but not in the presence, of indomethacin plus ADMA. Elevation of intraluminal pressure increased myogenic tone, which was reduced in arteries from ZDF rats. Conclusion The present findings show that flow evokes endothelium‐dependent EDHF‐type vasodilation involving BK Ca channels in penile arteries. Flow‐evoked vasodilation is reduced and only of EDHF‐type in penile arteries from type 2 diabetic rats suggesting modulation of this pathway may restore endothelial function and preserve erection in diabetes. Schjorring O, Kun A, Flyvbjerg A, Kirkeby HJ, Jensen JB, and Simonsen U. Flow‐evoked vasodilation is blunted in penile arteries from Zucker diabetic fatty rats. J Sex Med 2012;9:1806–1817. |
| Databáze: | OpenAIRE |
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