Circadian rhythms in Per1, PER2 and Ca2+ of a solitary SCN neuron cultured on a microisland
| Autor: | Sato Honma, Ken-ichi Honma, Yoshiaki Oda, Ryosuke Enoki, Yoshihiro Hirata, Kaori Kuribayashi-Shigetomi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Calcium metabolism endocrine system Multidisciplinary Chemistry Cell lcsh:R lcsh:Medicine Cell biology PER2 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Single-cell analysis nervous system medicine lcsh:Q Neuron Circadian rhythm lcsh:Science 030217 neurology & neurosurgery Intracellular PER1 |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-54654-5 |
| Popis: | Circadian rhythms in Per1, PER2 expression and intracellular Ca2+ were measured from a solitary SCN neuron or glial cell which was physically isolated from other cells. Dispersed cells were cultured on a platform of microisland (100–200 μm in diameter) in a culture dish. Significant circadian rhythms were detected in 57.1% for Per1 and 70.0% for PER2 expression. When two neurons were located on the same island, the circadian rhythms showed desynchronization, indicating a lack of oscillatory coupling. Circadian rhythms were also detected in intracellular Ca2+ of solitary SCN neurons. The ratio of circadian positive neurons was significantly larger without co-habitant of glial cells (84.4%) than with it (25.0%). A relatively large fraction of SCN neurons generates the intrinsic circadian oscillation without neural or humoral networks. In addition, glial cells seem to interrupt the expression of the circadian rhythmicity of intracellular Ca2+ under these conditions. |
| Databáze: | OpenAIRE |
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