Immunosuppression of in vivo and in vitro lymphocyte responses in swine induced by Trichinella spiralis or excretory-secretory antigens of the parasite
| Autor: | M. Gerenčer, Ivica Valpotić, M. Franković, D. Rapić, A. Marinculic |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Swine Lymphocyte Trichinella spiralis Antibodies Helminth chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Trichinosis Lymphocyte Activation Antigen In vivo medicine Immune Tolerance Animals Lymphocytes Swine Diseases Immunity Cellular General Veterinary biology Pokeweed mitogen Trichinellosis General Medicine medicine.disease biology.organism_classification medicine.anatomical_structure Concanavalin A Peripheral blood lymphocyte Antigens Helminth Immunology biology.protein Parasitology Female |
| Zdroj: | Veterinary parasitology. 44(3-4) |
| ISSN: | 0304-4017 |
| Popis: | The in vivo and in vitro effects of Trichinella spiralis excretory-secretory (ES) antigens on porcine peripheral blood lymphocyte (PBL) responses induced with mitogens (phytohemagglutinin, PHA; concanavalin A, Con A; pokeweed mitogen, PWM) or unrelated antigen (Protein A) were studied to determine whether ES antigens depress lymphocyte responses in experimental swine trichinosis, and/or if this response was manifested after lymphocytes from infected pigs had been pretreated with ES antigens. Additionally, the range of inhibition of lymphocyte responses was tested in parasite-free pigs using different doses of ES antigens and compared with the responsiveness of control cultures from the same animals. The responses of lymphocytes from pigs inoculated with 4 x 10(3) muscle larvae (ML) were strongly depressed (P0.05) at post-inoculation days (PID) 7 (after stimulation with PHA), 14, 35 (Con A or PWM), and 49 (PWM). At PID 56 and 63 the lymphocytes from T. spiralis-infected pigs responded better (P0.05) to all three mitogens than those from non-infected controls. After 7 weeks post-inoculation, PBL which were pretreated with 10 or 250 micrograms ml-1 of ES antigens showed significantly weaker (P0.05, P0.001) responses to PWM or PHA, respectively, than those from non-infected animals. The responsiveness of lymphocytes from both groups of pigs to Protein A was not affected by the pretreatment with ES antigens in vitro. The responses of lymphocytes from the parasite-free pigs induced by PHA, PWM or Protein A were strongly depressed (P0.01) after in vitro pretreatment regardless of the dose of ES antigens (5, 10, 15, or 20 micrograms ml-1) applied. |
| Databáze: | OpenAIRE |
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