A transcriptional reference map of defence hormone responses in potato
| Autor: | Pete E. Hedley, Jill Middlefell Williams, Lea Wiesel, Jayne L. Davis, Linda Milne, Paul R. J. Birch, Miriam B. Herold, Vanesa Redondo Fernandez, Adrian C. Newton, Ingo Hein, Jenny Morris, Brian Harrower, Eleanor M. Gilroy |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
2. Zero hunger
Multidisciplinary Methyl jasmonate Abiotic stress Gene Expression Profiling Jasmonic acid Computational Biology Reproducibility of Results food and beverages Molecular Sequence Annotation Biology Article Gene expression profiling chemistry.chemical_compound Plant Growth Regulators chemistry Biochemistry Gene Expression Regulation Plant Gene expression Jasmonate Databases Nucleic Acid Transcriptome Abscisic acid Salicylic acid Solanum tuberosum |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep15229 |
| Popis: | Phytohormones are involved in diverse aspects of plant life including the regulation of plant growth, development and reproduction, as well as governing biotic and abiotic stress responses. We have generated a comprehensive transcriptional reference map of the early potato responses to exogenous application of the defence hormones abscisic acid, brassinolides (applied as epibrassinolide), ethylene (applied as the ethylene precursor aminocyclopropanecarboxylic acid), salicylic acid and jasmonic acid (applied as methyl jasmonate). Of the 39000 predicted genes on the microarray, a total of 2677 and 2473 genes were significantly differentially expressed at 1 h and 6 h after hormone treatment, respectively. Specific marker genes newly identified for the early hormone responses in potato include: a homeodomain 20 transcription factor (DMG400000248) for abscisic acid; a SAUR gene (DMG400016561) induced in epibrassinolide treated plants; an osmotin gene (DMG400003057) specifically enhanced by aminocyclopropanecarboxylic acid; a gene weakly similar to AtWRKY40 (DMG402007388) that was induced by salicylic acid; and a jasmonate ZIM-domain protein 1 (DMG400002930) which was specifically activated by methyl jasmonate. An online database has been set up to query the expression patterns of potato genes represented on the microarray that can also incorporate future microarray or RNAseq-based expression studies. |
| Databáze: | OpenAIRE |
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