SOX-10 staining in dermal scars
| Autor: | Emily Behrens, Michelle Tarbox, Nerissa C D’Silva, Brooke Walterscheid, Philip D. Watkins, William D. Boothe |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male endocrine system Pathology medicine.medical_specialty Histology Skin Neoplasms Scars Antigens Differentiation Myelomonocytic Dermatology Stain Pathology and Forensic Medicine 030207 dermatology & venereal diseases 03 medical and health sciences Cicatrix 0302 clinical medicine MART-1 Antigen Antigens CD medicine Humans Histiocyte Aged Retrospective Studies Desmoplastic melanoma Aged 80 and over Staining and Labeling CD68 business.industry SOXE Transcription Factors Melanoma Histiocytes Dermis Middle Aged medicine.disease Immunohistochemistry Staining 030220 oncology & carcinogenesis embryonic structures Carcinoma Squamous Cell Female medicine.symptom business |
| Zdroj: | Journal of cutaneous pathologyREFERENCES. 46(8) |
| ISSN: | 1600-0560 |
| Popis: | Background Positive staining for SOX10 and the S100 protein are often used in the evaluation of challenging melanocytic neoplasms including melanoma in patient samples. SOX-10 positivity of non-melanocytes in re-excision specimen could complicate the evaluation of invasive melanoma with an invasive desmoplastic component. Therefore, quantifiable data regarding the positivity of SOX-10 in scars will help dermatopathologists to better identify false positive staining. Methods A retrospective analysis was performed on 50 re-excision specimens from 2013 to 2017, with a diagnosis of squamous cell carcinoma (SCC) or squamous cell carcinoma in situ (SCCIS). Blocks of re-excision specimens containing scars were stained for SOX-10; results were evaluated by a board-certified dermatopathologist. The sum of the five highest numbers of high-power field (HPF) counts as a proxy for "SOX-10 stain factor," and cell morphological features were analyzed. MART-1 and CD68 immunohistochemical staining was performed to study possible lineage of these SOX-10 positive cells. Results All 50 specimens showed varying degrees of SOX-10 positivity for histiocytes. SOX-10 positive histiocytes were present in 86% of re-excision scar tissues, of which 71.3% had spindle-shaped or angulated nuclei, and 61.8% had nuclear sizes larger than typical lymphocytes (7 μm). Within the same area of scars, CD68 staining was floridly positive, where as MART-1 staining was overwhelmingly negative. Conclusions This study illustrates a potential diagnostic pitfall of using SOX-10 to evaluate re-excision specimens of melanocytic neoplasms and also suggests a previously undescribed staining pattern in scars of SOX-10 positive cells that are not melanocytes. We postulate that such SOX-10 positive cells may represent a small fraction of histiocytes routinely found in scar tissue. |
| Databáze: | OpenAIRE |
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