Toward noninvasive assessment of flap viability with time-resolved diffuse optical tomography: a preclinical test on rats
| Autor: | Antonio Pifferi, Marion Aribert, Georges Bettega, Laura Di Sieno, Michel Berger, Henri Grateau, Jean-Luc Coll, Alberto Dalla Mora, Cynthia Hamou, Lionel Hervé, Jean-Marc Dinten, Davide Contini, Anne Planat-Chrétien, Agathe Puszka |
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| Přispěvatelé: | Dipartimento di Fisica, ICT Institute of Politecnico di Milano, Hôpital d'Annecy, Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Imagerie et Systèmes d'Acquisition (LISA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre hospitalier universitaire de Grenoble (CHU de Grenoble), CHU Grenoble, Istituto di Fotonica e Nanotecnologie (IFN), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Consiglio Nazionale delle Ricerche [Roma] (CNR) |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging image reconstruction techniques Biomedical Engineering tomography 01 natural sciences Surgical Flaps Reconstruction surgery 010309 optics Biomaterials 03 medical and health sciences 0302 clinical medicine Abdominal flaps 0103 physical sciences Occlusion medicine Image Processing Computer-Assisted Animals Tomography Optical Rats Wistar Venous occlusion business.industry image reconstruction techniques light propagation in tissues time-resolved imaging time-resolved imaging Atomic and Molecular Physics and Optics Diffuse optical imaging Electronic Optical and Magnetic Materials Surgery Rats 030220 oncology & carcinogenesis Female Tomography business Perfusion Biomedical engineering light propagation in tissues |
| Zdroj: | Journal of Biomedical Optics Journal of Biomedical Optics, 2016, 21 (2), pp.025004 ⟨10.1117/1.JBO.21.2.025004⟩ Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2016, 21 (2), pp.025004 ⟨10.1117/1.JBO.21.2.025004⟩ |
| ISSN: | 1560-2281 1083-3668 |
| DOI: | 10.1117/1.JBO.21.2.025004⟩ |
| Popis: | International audience; The noninvasive assessment of flap viability in autologous reconstruction surgery is still an unmet clinical need. To cope with this problem, we developed a proof-of-principle fully automatized setup for fast time-gated diffuse optical tomography exploiting Mellin–Laplace transform to obtain three-dimensional tomographic reconstructions of oxy- and deoxy-hemoglobin concentrations. We applied this method to perform preclinical tests on rats inducing total venous occlusion in the cutaneous abdominal flaps. Notwithstanding the use of just four source-detector couples, we could detect a spatially localized increase of deoxyhemoglobin following the occlusion (up to 550 μM in 54 min). Such capability to image spatio-temporal evolution of blood perfusion is a key issue for the noninvasive monitoring of flap viability. |
| Databáze: | OpenAIRE |
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