Tyrosine hydroxylase assay: a bioassay for aryl hydrocarbon receptor-active compounds based on tyrosine hydroxylase promoter activation

Autor:	Saeko Uruno, Seiko Yoshimura, Mitsuko Ishihara-Sugano, Eiichi Akahoshi, Satoshi Itoh
Rok vydání:	2012
Předmět:	chemistry.chemical_classification Reporter gene biology Tyrosine hydroxylase Tyrosine 3-Monooxygenase Health Toxicology and Mutagenesis Neurotoxicity Toxicology Aryl hydrocarbon receptor medicine.disease Mice Hydrocarbon Promoter activity chemistry Biochemistry Receptors Aryl Hydrocarbon Cell Line Tumor biology.protein medicine Bioassay Animals Receptor Promoter Regions Genetic
Zdroj:	Toxicology mechanisms and methods. 22(6)
ISSN:	1537-6524
Popis:	Tyrosine hydroxylase (TH) assay is a novel bioassay for screening aryl hydrocarbon receptor (AhR)-active compounds with diverse toxicities. The TH assay employs reporter genes to evaluate TH promoter activity, which is linked to dopamine synthesis and regulated by the AhR-aryl hydrocarbon receptor responsive element-III (AHRE-III)-mediated pathway. The toxic equivalency factors for higher chlorinated dioxin congeners in the TH assay (TH-TEF) exhibit the same tendency as those for the WHO-TEF, indicating that the activity of the TH assay is consistent with that of existing methods. Furthermore, other chemicals, such as lower chlorinated dioxin congeners that were minimally investigated in the past could be detected preferentially by this assay.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::269bdfc32fce599aab6ea383fe681b29 https://pubmed.ncbi.nlm.nih.gov/22394336 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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