Supplementary Figure 2 from Sorafenib-Mediated Targeting of the AAA+ ATPase p97/VCP Leads to Disruption of the Secretory Pathway, Endoplasmic Reticulum Stress, and Hepatocellular Cancer Cell Death

Autor: Eric Chevet, Jean-Frédéric Blanc, Paulette Bioulac-Sage, Charles Balabaud, Jean Rosenbaum, Jeremy C. Simpson, Brigitte Le Bail, Claire Castain, Daniela Arma, Esther Marza, Mariana G. Bexiga, Said Taouji, Arisa Higa, Ping Yi
Rok vydání: 2023
DOI: 10.1158/1535-7163.22496404.v1
Popis: PDF file - 510K, Figure S2. (A) HepG2 cells were either not treated or treated with 10 μM Sorafenib for 4 h, after which they were either fixed or Sorafenib removed from cells and fresh medium added for a further 2 h. Cells were then stained with anti Giantin (green). Scale bars correspond to 25 μm. (B) The number of cells containing fragmented Golgi complex was quantified. Between 200 and 400 cells were analyzed for each condition and results are represented as the means � SD. Student's t-test *: p < 0.03, compared to control, #: p < 0.03 HepG2 mock transfected compared to IRE1-DN transfected cells.
Databáze: OpenAIRE