A tungsten supplemented diet attenuates bacterial translocation in chronic portal hypertensive and cholestatic rats: role of xanthine dehydrogenase and xanthine oxidase
| Autor: | S. Takahashi, G Feierl, Maria Anna Pabst, A.-M. Kuesz, Michael E. Höllwarth, Hüseyin Özbey, G. Schimpl |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Xanthine Oxidase medicine.medical_specialty Xanthine Dehydrogenase Spleen Biology Article Tungsten Rats Sprague-Dawley Jejunum chemistry.chemical_compound Internal medicine Hypertension Portal medicine Animals Mesenteric lymph nodes Xanthine oxidase Barrier function Cholestasis Gastroenterology medicine.disease Pathophysiology Rats medicine.anatomical_structure Endocrinology Xanthine dehydrogenase chemistry Bacterial Translocation Dietary Supplements Portal hypertension |
| Zdroj: | Gut. 45:904-910 |
| ISSN: | 0017-5749 |
| DOI: | 10.1136/gut.45.6.904 |
| Popis: | BACKGROUND—Bacterial translocation (BT) plays a major role in the pathophysiological process of spontaneous infections in portal hypertension (PH) and cholestatic jaundice. The major mechanisms promoting BT in experimental animal models are the disruption of the intestinal ecological equilibrium and disruption of the intestinal mucosal barrier. The enzymes xanthine dehydrogenase (XD) and xanthine oxidase (XO) are often implicated as a significant source of oxidants which have a major impact on the impairment of intestinal barrier function. AIM—To investigate the incidence of BT in rats with PH and obstructive jaundice, and to evaluate the impact of XD and XO. METHODS—Animals were subjected to sham laparotomy (SL), PH by calibrated stenosis of the portal vein, and common bile duct ligation (CBDL). They were fed either a standard pellet diet or a tungsten supplemented molybdenum-free diet. Four weeks after the operative procedure, intestinal colonisation and BT to portal vein, vena cava, mesenteric lymph nodes, liver, and spleen were determined. Intestinal XD and XO activity were measured enzymatically and histochemically. RESULTS—Significant (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|