Regulation of the Na+/K+-ATPase Ena1 expression by calcineurin/Crz1 under high pH stress : a quantitative study
| Autor: | Silvia Petrezsélyová, Ester Vilaprinyo, Lynne Yenush, María López-Malo, M. Carmen Marqués, Alicia Roque, Albert Serra-Cardona, Rui Alves, Joaquín Ariño, David Canadell |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Alkaline pH stress Molecular biology Microfluidics lcsh:Medicine Gene Expression Yeast and Fungal Models Protein accumulation Biochemistry Physical Chemistry mRNA levels Gene Expression Regulation Fungal Cation homeostasis Regulation of gene expression Regulatory Elements Transcriptional lcsh:Science Quantitative analysis Promoter Regions Genetic Recombinant proteins Multidisciplinary Calcineurin Messenger RNA Hydrogen-Ion Concentration Recombinant Proteins Cell biology Nucleic acids DNA-Binding Proteins Denaturation Chemistry Protein Transport Protein phosphatase Physical Sciences Saccharomyces Cerevisiae Engineering and Technology Fluidics Signal transduction Sodium-Potassium-Exchanging ATPase Research Article Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Phosphatase DNA transcription Active Transport Cell Nucleus Biology Research and Analysis Methods Gene Expression Regulation Enzymologic 03 medical and health sciences Saccharomyces Model Organisms Stress Physiological Cations Transcription rate BIOQUIMICA Y BIOLOGIA MOLECULAR Genetics Transcription factors Gene Regulation Na+/K+-ATPase Transcription factor Crz1 Transcription factor Ions Cell Nucleus Binding Sites 030102 biochemistry & molecular biology Biology and life sciences Organisms Genetically Modified lcsh:R Organisms Fungi Proteins biology.organism_classification Yeast Regulatory Proteins RNA denaturation 030104 developmental biology Molecular biology techniques RNA lcsh:Q Transcription Factors |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname PLoS ONE Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Repositorio Abierto de la UdL Universitad de Lleida Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) PLoS ONE, Vol 11, Iss 6, p e0158424 (2016) RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Popis: | [EN] Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling pathways, including that mediated by the calcium-regulatable protein phosphatase calcineurin and its downstream transcription factor Crz1. We present here a quantitative study of the expression of Ena1 in response to alkalinization of the environment and we analyze the contribution of Crz1 to this response. Experimental data and mathematical models substantiate the existence of two stress-responsive Crz1-binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early response of the ENA1 promoter is about 60%. The models suggest the existence of a second input with similar kinetics, which would be likely mediated by high pH-induced activation of the Snf1 kinase. This work was supported by grants BFU2011-30197-C3-01, BFU2014-54591-C2-1-P and EUI2009-04147 (SysMo2) to JA. (Ministry of Industry and Competitivity, Spain, and Fondo Europeo de Desarrollo Regional [FEDER]). JA is the recipient of an Ajut 2014SGR-4 award (Generalitat de Catalunya). DC was recipient of a predoctoral fellowship from the Spanish Ministry of Education. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Databáze: | OpenAIRE |
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