Mesangial Cells Cultured from Pregnant Rats Display Reduced Reactivity to Angiotensin II: the Role of Relaxin, Nitric Oxide and AT2 Receptor
| Autor: | Clévia Santos Passos, Lucimeire Nova Carvalho, Priscila Cardoso Cristovam, Mirian A. Boim |
|---|---|
| Přispěvatelé: | Universidade Federal de São Paulo (UNIFESP) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Receptors Peptide Physiology Pyridines Stimulation Vasodilation Angiotensin II Type 2 Receptor Blockers AT2 receptor Nitric Oxide Receptor Angiotensin Type 2 Calcium in biology Nitric oxide Receptors G-Protein-Coupled chemistry.chemical_compound Mesangial cells Pregnancy Internal medicine Renin–angiotensin system medicine Animals Calcium Signaling Rats Wistar Receptor Intracellular calcium Cells Cultured Relaxin Chemistry Angiotensin II Imidazoles Rats Endocrinology NG-Nitroarginine Methyl Ester Mesangial Cells Female Nitric Oxide Synthase hormones hormone substitutes and hormone antagonists |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| Popis: | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundacao Oswaldo Ramos (FOR) Background/Aims: Pregnancy is characterized by vasodilatation and increased glomerular filtration rate (GFR), despite overstimulation of the renin angiotensin system (RAS). the mesangial cells (MCs) influences GFR and when cultured from pregnant rats displays refractoriness to Ang II. We evaluated the role of relaxin (RLX) and its receptor (RXFP1), nitric oxide (NO) and the AT2 receptor in this response. Methods: MCs cultured from kidneys of virgin (V) and pregnant (P) Wistar rats were treated with RLX or AT2 receptor blocker PD123319 or NO synthase inhibitor L-NAME. After 24 hr, intracellular calcium concentration ([Ca]i) was recorded before and after the addition of Ang II. Results: MCs from V group expressed AT2, RLX and RXFP1, whose levels were increased in P cells. Ang II induced a 150% increase in [Ca]i in the V cells and 85% (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|