Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans
| Autor: | David Ivor Gwynne, Frank Buxton, R W Davies |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
DNA Replication
Recombination Genetic Aspergillus biology Genes Fungal Genetic Complementation Test Genetic Vectors fungi Aspergillus niger General Medicine Arginine biology.organism_classification Molecular biology Aspergillus nidulans Transformation (genetics) Transformation Genetic Plasmid Shuttle vector Genetics Cosmid Ornithine Carbamoyltransferase Plasmids Southern blot |
| Zdroj: | Gene. 37:207-214 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(85)90274-4 |
| Popis: | A mutant of Aspergillus niger defective in ornithine transcarbamylase function was transformed with plasmids carrying a functional copy of the argB gene of Aspergillus nidulans after treatment of spheroplasts in the presence of polyethylene glycol and calcium ions. The plasmid pDG3 gave stable transformants at a frequency of 4 per microgram of input DNA. Southern blot analysis of DNA from transformants showed that pDG3 DNA had integrated into the A. niger chromosomes at a variety of locations. The transformants were phenotypically stable for many mitotic divisions. This procedure may potentially be used to insert any gene into the genome of A. niger. A cosmid shuttle vector, pDG1, for cloning in Aspergillus was also constructed. |
| Databáze: | OpenAIRE |
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