Expression of SH2D2A in T-cells is regulated both at the transcriptional and translational level
| Autor: | Anne Spurkland, Monika Forsbring, Øyvind Molberg, Vibeke Sundvold-Gjerstad, Karl Schenck, Hans Christian Aasheim, Kristin M. Kolltveit, Karoline W. Schjetne, B. Bogen, Stine Granum, Ke Zheng Dai, Finn-Eirik Johansen, Virginia Smith Shapiro |
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| Rok vydání: | 2008 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cytoplasm CD3 Complex Transcription Genetic Immunology Receptors Antigen T-Cell Priming (immunology) Stimulation Biology Peripheral blood mononuclear cell RNA Transport Cross-Priming Transcription (biology) Cyclic AMP Humans RNA Messenger Molecular Biology Gene Adaptor Proteins Signal Transducing Regulation of gene expression Sulfonamides T-cell receptor Models Immunological Signal transducing adaptor protein Isoquinolines Cyclic AMP-Dependent Protein Kinases Molecular biology Gene Expression Regulation Protein Biosynthesis Signal Transduction |
| Zdroj: | Molecular Immunology. 45:2380-2390 |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/j.molimm.2007.11.005 |
| Popis: | The T-cell specific adapter protein (TSAd) encoded by the SH2D2A gene is up-regulated in activated human CD4+ T-cells in a cAMP-dependent manner. Expression of SH2D2A is important for proper activation of T-cells. Here, we show that SH2D2A expression is regulated both at the transcriptional and translational level. cAMP signaling alone induces TSAd-mRNA expression but fails to induce increased TSAd protein levels. By contrast, TCR engagement provides signals for both TSAd transcription and translation. We further show that cAMP signaling can prime T-cells for a more prompt expression of TSAd protein upon TCR stimulation. Our study thus points to a novel mechanism for how cAMP signaling may modulate T-cell activation through transcriptional priming of resting cells. |
| Databáze: | OpenAIRE |
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