The Arf family G protein Arl1 is required for secretory granule biogenesis in Drosophila
| Autor: | Cláudia Rosa-Ferreira, Sean Munro, Isabel L. Torres |
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| Rok vydání: | 2014 |
| Předmět: |
Male
G protein Small G Protein Biology Clathrin symbols.namesake Animals Drosophila Proteins Secretory pathway Secretory Vesicles Membrane Proteins Signal transducing adaptor protein Cell Biology Golgi apparatus Protein Structure Tertiary Cell biology Transcription Factor AP-1 Protein Transport Secretory protein symbols biology.protein ADP-Ribosylation Factor 1 Drosophila Female Biogenesis Research Article |
| Zdroj: | Journal of Cell Science. |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.122028 |
| Popis: | The small G protein Arf like 1 (Arl1) is found at the Golgi complex, and its GTP-bound form recruits several effectors to the Golgi including GRIP-domain-containing coiled-coil proteins, and the Arf1 exchange factors Big1 and Big2. To investigate the role of Arl1, we have characterised a loss-of-function mutant of the Drosophila Arl1 orthologue. The gene is essential, and examination of clones of cells lacking Arl1 shows that it is required for recruitment of three of the four GRIP domain golgins to the Golgi, with Drosophila GCC185 being less dependent on Arl1. At a functional level, Arl1 is essential for formation of secretory granules in the larval salivary gland. When Arl1 is missing, Golgi are still present but there is a dispersal of adaptor protein 1 (AP-1), a clathrin adaptor that requires Arf1 for its membrane recruitment and which is known to be required for secretory granule biogenesis. Arl1 does not appear to be required for AP-1 recruitment in all tissues, suggesting that it is crucially required to enhance Arf1 activation at the trans-Golgi in particular tissues. |
| Databáze: | OpenAIRE |
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