Physicochemical properties of water-soluble polysaccharides from black cumin seeds
| Autor: | Malika Ayadi, Salma Cheikh-Rouhou, Hamadi Attia, Ines Trigui, Ali Bougatef, Hela Yaich, Christophe Blecker, Assaâd Sila |
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| Rok vydání: | 2018 |
| Předmět: |
Chemical Phenomena
DPPH medicine.medical_treatment 02 engineering and technology Polysaccharide Biochemistry Antioxidants chemistry.chemical_compound 0404 agricultural biotechnology Picrates Structural Biology Polysaccharides medicine Nigella sativa Sugar Molecular Biology chemistry.chemical_classification Carotene Biphenyl Compounds Temperature Water 04 agricultural and veterinary sciences General Medicine Nuclear magnetic resonance spectroscopy Galactan 021001 nanoscience & nanotechnology Glucuronic acid beta Carotene 040401 food science chemistry Pyranose Solubility Seeds 0210 nano-technology Oils Nuclear chemistry |
| Zdroj: | International journal of biological macromolecules. 117 |
| ISSN: | 1879-0003 |
| Popis: | In the present work, water-soluble polysaccharides were isolated from black cumin seeds. Polysaccharides were characterized by their carbohydrate composition, molecular weight, thermal stability and by FTIR, NMR spectroscopy and X-ray diffraction. The surface, the functional and the antioxidant properties of black cumin water-soluble polysaccharides (BCWSP) were also investigated. BCWSP consisted mainly of galacturonic acid (30.20%), glucuronic acid (17.66%) and neutral sugar (22.99%). BCWSP was composed of high peak molecular weight. The FTIR spectrum obtained for BCWSP showed two most important absorptions, at 1659 and 1085 cm−1, which corresponded to COO– of uronic acids and pyranose form, respectively. NMR spectroscopy data suggested that the BCWSP is probably a rhamnogalacturonan backbone with galactan and arabinan side chains. X-ray pattern revealed the semi-crystalline behavior of BCWSP. WHC and OHC of BCWSP were relatively high and varied with temperatures. The polysaccharide zeta potential was greatly affected by pH. Results indicated that the decrease of surface tension has influenced foaming and emulsifying capacities. The DPPH radical scavenging activity of the BCWSP was 63.25% at 1 mg/mL. The BCWSP displayed moderate reductive, β carotene bleaching and chelating abilities. Overall, our results suggested that BCWSP could be used as alternative additives in food and non-food products. |
| Databáze: | OpenAIRE |
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