Human cytomegalovirus UL97 kinase confers ganciclovir susceptibility to recombinant vaccinia virus
| Autor: | Karin Schneider, C Metzger, Anke Lüske, Detlef Michel, Thomas Mertens, H J Schlicht |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Ganciclovir
Human cytomegalovirus Transcription Genetic viruses Molecular Sequence Data Restriction Mapping Immunology Cytomegalovirus Guanosine Bone Neoplasms Vaccinia virus Viral Plaque Assay Biology Transfection Polymerase Chain Reaction Microbiology Virus law.invention Gene product Open Reading Frames chemistry.chemical_compound law Virology Tumor Cells Cultured medicine Humans Cloning Molecular DNA Primers Sequence Deletion Recombination Genetic Osteosarcoma Base Sequence medicine.disease Molecular biology Recombinant Proteins Phosphotransferases (Alcohol Group Acceptor) Open reading frame chemistry Insect Science DNA Viral Recombinant DNA Regression Analysis Vaccinia Research Article medicine.drug |
| Zdroj: | Journal of Virology. 68:8423-8427 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.68.12.8423-8427.1994 |
| Popis: | We analyzed whether the phosphotransferase encoded by the UL97 open reading frame of human cytomegalovirus (HCMV) alone is sufficient to confer ganciclovir (GCV) susceptibility to a foreign virus. Two vaccinia virus recombinants (T1 and A5) containing the UL97 open reading frames from a GCV-sensitive HCMV and from a GCV-resistant strain were constructed. T1 exhibited a GCV-sensitive phenotype in plaque reduction assays, whereas A5 did not. Moreover, T1-infected cell cultures showed a strongly increased incorporation of [14C]GCV triphosphate into macromolecular DNA, compared with recombinant A5 or vaccinia virus controls, which could be inhibited by the addition of guanosine. This shows that UL97 kinase is the only additional gene product required to make vaccinia virus susceptible to GCV, and guanosine seems to be one natural substrate for the enzyme. The system described here should be very helpful for fast and detailed functional analyses of UL97 mutations found in GCV-resistant HCMV isolates. |
| Databáze: | OpenAIRE |
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