Quantitation of plasma circulating DNA using quantitative PCR for the detection of hepatocellular carcinoma
| Autor: | Qi-gen Xie, Zhaohui Huang, Yanjun Zeng, Xiang Du, Dong Hua, Zhi-hong Cheng, Qiong-yao Wang, Xike Zhou, Yu Hu, Feng Wang |
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| Rok vydání: | 2011 |
| Předmět: |
Liver Cirrhosis
Male Cancer Research medicine.medical_specialty Pathology Cirrhosis Carcinoma Hepatocellular Biology Real-Time Polymerase Chain Reaction Gastroenterology Pathology and Forensic Medicine Metastasis chemistry.chemical_compound Internal medicine medicine Carcinoma Biomarkers Tumor Humans Survival rate Hepatitis Chronic Neoplasm Staging Hepatitis Liver Neoplasms General Medicine DNA Middle Aged medicine.disease Prognosis Survival Rate Real-time polymerase chain reaction Oncology chemistry ROC Curve Hepatocellular carcinoma Case-Control Studies Female |
| Zdroj: | Pathology oncology research : POR. 18(2) |
| ISSN: | 1532-2807 |
| Popis: | Circulating DNA is a potential biomarker for tumor diagnosis and prognosis. This study was aimed to quantify the circulating DNA in plasma from patients with hepatocellular carcinoma (HCC) using quantitative PCR and evaluate its potential clinical value. Blood samples were collected from 72 patients with HCC, 37 with liver cirrhosis or chronic hepatitis and 41 healthy volunteers. Plasma DNA was extracted and quantified by a real-time quantitative PCR method. The diagnostic and prognostic value of plasma DNA analysis for HCC was evaluated. DNA levels in the HCC plasma (median: 173 ng/mL) were significantly higher than those in the healthy controls (9 ng/mL) or control benign patients (46 ng/mL) (P |
| Databáze: | OpenAIRE |
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