Determination of DNA in densovirus-infected invertebrate cell line by flow cytometry
| Autor: | G. Devauchelle, P. Vago, J.-M. Quiot, J. P. Bureau, F. Odier |
|---|---|
| Přispěvatelé: | Unité mixte de recherche de pathologie comparée, Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
0303 health sciences
medicine.diagnostic_test Biology Cell cycle Virology Molecular biology Virus In vitro 3. Good health Flow cytometry [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Cell culture 030220 oncology & carcinogenesis medicine Ultrastructure Densovirus CYTOMETRIE EN FLUX Ecology Evolution Behavior and Systematics DNA ComputingMilieux_MISCELLANEOUS 030304 developmental biology |
| Zdroj: | Journal of Invertebrate Pathology Journal of Invertebrate Pathology, Elsevier, 1993, 62, pp.252-256 |
| ISSN: | 0022-2011 1096-0805 |
| Popis: | The DNA content of lepidopteran cell lines infected with densovirus was determined by flow cytometry. Vital DNA production was monitored and in vitro viral cytopathogenic effects were analyzed. The increased fluorochrome binding was linked to an increase in viral DNA content and to alteration in the nucleus structure observed under electron microscopy. The information provided by flow cytometry was complemented by ultrastructural studies. |
| Databáze: | OpenAIRE |
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