Presence of Metastasis-associated Protein 1 in Sertoli Cells is Required for Proper Contact Between Sertoli Cells and Adjacent Germ Cells

Autor: Wei Li, He Wang, Hang Sun, Rong Liu, Bo Yang, Chuchao Zhu
Rok vydání: 2013
Předmět:
Male
Transcription
Genetic
Somatic cell
Gene Expression
Cell Communication
Mice
Calcium Channels
N-Type
Gene expression
Phosphorylation
Anchoring junction
Azoospermia
Mitogen-Activated Protein Kinase 1
Mice
Inbred BALB C
Gene knockdown
Sertoli Cell-Only Syndrome
Transferrin
Adherens Junctions
Middle Aged
Sertoli cell
Immunohistochemistry
Neoplasm Proteins
Cell biology
medicine.anatomical_structure
Gene Knockdown Techniques
Adult
Alkylating Agents
Cell signaling
Urology
Fatty Acid-Binding Proteins
Histone Deacetylases
Tight Junctions
Calcium Channels
Q-Type
Sertoli cell-only syndrome
Young Adult
medicine
Animals
Humans
Hedgehog Proteins
Spermatogenesis
Busulfan
Sertoli Cells
business.industry
Calcium Channels
P-Type
Oligospermia
medicine.disease
Coculture Techniques
Repressor Proteins
Germ Cells
Immunology
Trans-Activators
business
Proto-Oncogene Proteins c-akt
Zdroj: Urology. 81:66-73
ISSN: 0090-4295
DOI: 10.1016/j.urology.2012.07.034
Popis: Objective To investigate whether the normal expression of metastasis-associated protein 1 (MTA1) in Sertoli cells (SCs) is associated with adjacent germ cells (GCs) and to provide the functional relevance of MTA1 in this somatic cell. Methods The expression pattern of MTA1 in the SCs of impaired human spermatogenesis was determined using immunohistochemistry. The effect of the depletion of GCs on the expression of MTA1 in isolated SCs was evaluated using reverse transcriptase polymerase chain reaction in murine testes treated with busulphan. Finally, using multiple assays, the functional investigation of MTA1 by its specific knockdown was performed in SC-GC co-cultures. Results SCs were negatively immunolabeled in the tubules with impaired spermatogenesis. Depletion of murine GCs by treatment with busulphan resulted in a dramatic decrease of the MTA1 transcripts level in the isolated SCs on the 15th day of treatment and thereafter had totally abolished MTA1 expression by the 30th day of treatment, respectively. The addition of isolated round spermatids into SC culture could partially elevate MTA1 expression in the latter. Furthermore, MTA1 is crucial to maintain the GC nursery function and normal anchoring junction formation in SCs because ablation of MTA1 by siRNA induced extensive defects of genes related to SC homeostasis. Conclusion We propose a novel role for SC-expressing MTA1, which is determined by the presence of surrounding GCs, in mediating the crosstalk between SCs and GCs by influencing a broad spectrum of gene changes.
Databáze: OpenAIRE
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