Conversion of a Thiol Precursor into Aroma Compound 4-mercapto-4-methyl-2-pentanone Using Microbial Cell Extracts
| Autor: | Chiao-Yen Liu, Hsuan-Ju Lin, Yu-Hsuan Wu, Pang-Hung Hsu, Yu-Ting Lee, Tze-Chia Lin, Hong-Ting Victor Lin, Jung-Lee Lin, Hao-Kai Li, Chi-Fon Chang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
aroma compounds 4-mercapto-4-methyl-2-pentanone β-lyase precursors fermentation Shewanella putrefaciens Chemical structure Fermentation industries. Beverages. Alcohol 030106 microbiology Plant Science Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences chemistry.chemical_compound Aroma compound Aroma chemistry.chemical_classification TP500-660 Chromatography biology Chemistry food and beverages biology.organism_classification Yeast 030104 developmental biology Thiol Fermentation Bacteria Food Science |
| Zdroj: | Fermentation, Vol 7, Iss 129, p 129 (2021) Fermentation; Volume 7; Issue 3; Pages: 129 |
| ISSN: | 2311-5637 |
| Popis: | 4-Mercapto-4-methyl-2-pentanone (4MMP), a high-impact aroma compound with the box tree and black currant flavors was first identified in wines and could be released by microbial cysteine-S-conjugate β-lyases from its precursors. In this study, various yeasts and bacteria encoding β-lyases were selected to examine their β-lyase activities. A thiol precursor of 4MMP, cysteine-conjugate of 4MMP (cys-4MMP), was synthesized with a purity of >95% in a relatively environmentally friendly approach, and its chemical structure was confirmed by nuclear magnetic resonance spectroscopy. The β-lyase activities of the crude cell extract from the bacteria and yeast strains for different substrates were examined using a colorimetric method. Shewanella putrefaciens cell extract exhibited the highest β-lyase activity for all tested substrates. Additionally, the optimum pH and temperature for their β-lyase activities were determined. To monitor the conversion efficiency of precursor cys-4MMP to 4MMP, liquid chromatography-mass spectrometry was used. Our data indicate that selected bacteria and yeasts could convert cys-4MMP into 4MMP, and S. putrefaciens exhibited the best conversion yield. This study demonstrated the potential use of microbial cell extracts to produce sulfur-containing aroma compounds such as 4MMP. |
| Databáze: | OpenAIRE |
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