Na,K-ATPase is decreased in hippocampus of kainate-lesioned rats
| Autor: | Auday A. Maki, William R. Anderson, J. E. Franck, William L. Stahl |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Kainic acid Alpha (ethology) Hippocampus Kainate receptor In situ hybridization Biology Ouabain chemistry.chemical_compound Internal medicine medicine Animals RNA Messenger Na+/K+-ATPase In Situ Hybridization Injections Intraventricular Kainic Acid Pyramidal Cells Rats Endocrinology medicine.anatomical_structure nervous system Neurology chemistry Autoradiography Neurology (clinical) Sodium-Potassium-Exchanging ATPase Pyramidal cell medicine.drug |
| Zdroj: | Epilepsy Research. 17:221-231 |
| ISSN: | 0920-1211 |
| DOI: | 10.1016/0920-1211(94)90052-3 |
| Popis: | The effects of intraventricular injection of kainic acid on the Na,K-ATPase (Na,K pump) were examined in discrete pyramidal cell regions of rat hippocampus. [3H]Ouabain binding was used to quantitate Na,K-ATPase catalytic subunits and in situ hybridization was used to determine Na,K-ATPase mRNA levels. Large decreases were found in both [3H]ouabain binding and alpha 3 isoform mRNA in hippocampus areas, especially in the CA3 pyramidal cell layer, which sustains heavy cell losses as a result of bilateral, intraventricular injection of kainic acid. Substantial decreases in the high affinity component of ouabain binding and in the alpha 3 isoform mRNA (but not isoforms for other Na,K-ATPase subunits) were also observed in the CA1 region of hippocampus, an area preserved in this model. High affinity [3H]ouabain binding was decreased 25-33% in the stratum pyramidale and stratum radiatum after treatment with kainic acid, and alpha 3 mRNA was decreased by 26-50%. To further characterize the decrease in alpha 3 mRNA, animals were killed at 1, 2, and 3 weeks after injection of kainate and results show a large decrease in alpha 3 mRNA only at 2 weeks recovery time. While the pathology underlying temporal lobe epilepsy is unclear, changes in the Na,K-ATPase may be involved in abnormal firing characteristics of cells in epileptic tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|