LXN deficiency regulates cytoskeleton remodelling by promoting proteolytic cleavage of Filamin A in vascular endothelial cells
| Autor: | Guozhang He, Shuang Kan, Shaohua Xu, Ming Chen, Wei Shu, Rong Li, Xuchen Sun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Filamins Cell Nerve Tissue Proteins Vascular permeability Vasodilation Filamin Mice 03 medical and health sciences Apolipoproteins E 0302 clinical medicine Human Umbilical Vein Endothelial Cells medicine Animals Humans FLNA Cytoskeleton Aorta Gene knockdown Chemistry Original Articles Cell Biology endothelial cells Cell biology Mice Inbred C57BL Endothelial stem cell 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Blood Circulation Proteolysis cytoskeleton remodelling Molecular Medicine Original Article Endothelium Vascular Stress Mechanical atherosclerosis LXN laminar shear stress |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/jcmm.16685 |
| Popis: | Endothelial cells (ECs) respond to blood shear stress by changing their morphology is important for maintaining vascular homeostasis. Studies have documented a relationship between endothelial cell shape and the stress flow, and however, the mechanism underlying this cytoskeletal rearrangement due to shear stress remains uncertain. In this paper, we demonstrate that laminar shear stress (LSS) significantly reduces latexin (LXN) expression in ECs. By using siRNA and cell imaging, we demonstrated that LXN knockdown results in the morphologic change and F‐actin remodelling just like what LSS does in ECs. We further demonstrate that LXN interacts with Filamin A (FLNA) and regulates FLNA proteolytic cleavage and nuclei translocation. By constructing LXN‐/‐ mice and ApoE‐/‐LXN‐/‐ double knockout mice, we evaluated the effect of LXN knockout on aortic endothelium damage in mice. We found that LXN deficiency significantly improves vascular permeability, vasodilation and atherosclerosis in mice. Our findings provide confident evidence, for the first time, that LXN is a novel regulator for morphological maintenance of ECs, and LXN deficiency has a protective effect on vascular homeostasis. This provides new strategies and drug targets for the treatment of vascular diseases. |
| Databáze: | OpenAIRE |
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