Studying Tunicata WBR Using Botrylloides anceps

Autor:	Arzu Karahan, Esra Öztürk, Berivan Temiz, Simon Blanchoud
Rok vydání:	2022
Předmět:	Research Animals Urochordata Phylogeny Research Article
Zdroj:	Methods in Molecular Biology ISBN: 9781071621714 Methods Mol Biol
DOI:	10.1007/978-1-0716-2172-1_16
Popis:	Tunicates are marine filter-feeding invertebrates that can be found worldwide and which are the closest phylogenetic group to the vertebrates (Craniata). Of particular interest, colonial tunicates are the only known chordates that can undergo Whole-Body Regeneration (WBR) via vascular budding. In Botrylloides anceps, a fully functional adult regenerates from a fragment of the vascular system in around 2 weeks after amputation. In this chapter, we present protocols to collect B. anceps colonies, confirm their species, breed them in the lab, monitor WBR and perform histological staining on cryosections.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25ef7ae0fd52f5b896ad894735407490 https://doi.org/10.1007/978-1-0716-2172-1_16 Zobrazit plný text záznamu