Experimental generation of a tissue-engineered functional and vascularized trachea
| Autor: | Fred Hofmann, Bettina Giere, Johanna Schanz, Heike Mertsching, Thorsten Walles, Michael Hofmann, Paolo Macchiarini |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2004 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Swine Myocytes Smooth Muscle Matrix (biology) Chondrocyte Tissue culture Tissue engineering Animals Medicine Viability assay Progenitor cell Cells Cultured Bioartificial Organs Tissue Engineering business.industry Stem Cells Immunohistochemistry Trachea Endothelial stem cell medicine.anatomical_structure Respiratory epithelium Surgery Cardiology and Cardiovascular Medicine business |
| Zdroj: | The Journal of Thoracic and Cardiovascular Surgery. 128:900-906 |
| ISSN: | 0022-5223 |
| DOI: | 10.1016/j.jtcvs.2004.07.036 |
| Popis: | Objective We sought to grow in vitro functional smooth muscle cells, chondrocytes, and respiratory epithelium on a biologic, directly vascularized matrix as a scaffold for tracheal tissue engineering. Methods Ten- to 15-cm–long free jejunal segments with their own vascular pedicle were harvested and acellularized from donor pigs (n = 10) and used as a vascular matrix. Autologous costal chondrocytes, smooth muscle cells, and respiratory epithelium and endothelial progenitor cells were first cultured in vitro and then disseminated on the previously acellularized vascular matrix. Histologic, immunohistologic, molecular imaging, and Western blotting studies were then performed to assess cell viability. Results The endothelial progenitor cells re-endothelialized the matrix to such an extent that endothelial cell viability was uniformly documented through 2-(18F)-fluoro-2′-deoxyglucose positron emission tomography. This vascularized scaffold was seeded with functional (according to Western blot analysis) smooth muscle cells and successfully reseeded with viable ciliated respiratory epithelium. Chondrocyte growth and production of extracellular cartilaginous matrix was observed as soon as 2 weeks after their culture. Conclusions The fundamental elements for a bioartificial trachea were successfully engineered in vitro in a direct vascularized 10- to 15-cm–long bioartificial matrix. Future experimental work will be directed to give them a 3-dimensional aspect and a biomechanical profile of a functioning trachea. |
| Databáze: | OpenAIRE |
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