An open-label dosing study to evaluate the safety and effects of a dietary plant-derived polysaccharide supplement on the N-glycosylation status of serum glycoproteins in healthy subjects
| Autor: | Edward Tarelli, John S. Axford, Owen Fraser, Martin Bland, Azita Alavi |
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| Rok vydání: | 2011 |
| Předmět: |
dietary plant polysaccharides
Adult Male medicine.medical_specialty Glycosylation Medicine (miscellaneous) N-glycans Pilot Projects Biology Polysaccharide sialylation chemistry.chemical_compound N-linked glycosylation Polysaccharides glycomodifications Internal medicine Dietary Carbohydrates medicine Humans Serum glycosylation Adverse effect Glycoproteins chemistry.chemical_classification Nutrition and Dietetics Dose-Response Relationship Drug Middle Aged Plants dietary fiber Dose–response relationship Endocrinology chemistry Dietary Supplements Toxicity Immunology Original Article Female Glycoprotein |
| Zdroj: | European Journal of Clinical Nutrition |
| ISSN: | 1476-5640 0954-3007 |
| DOI: | 10.1038/ejcn.2010.263 |
| Popis: | Background: The functional role of dietary carbohydrates in nutrition is one of the most complex and at times controversial areas in nutritional science. In-vitro and in-vivo studies suggest that certain dietary saccharide biopolymers can have bifidogenic and or immunomodulatory effects, and that some could represent preferential substrates or precursors that can impact cellular glycosylation. Objective: Examine the impact of oral ingestion of a standardized dietary plant-derived polydisperse polysaccharide supplement (Advanced Ambrotose powder (AA)) on the N-glycosylation status of serum glycoproteins in a cohort of healthy individuals. Design: An open-label study was carried out. This study was in two phases: pilot study (n=6 individuals) to assess safety and dose, and a larger study (n=12) to evaluate specific glycosylation changes. Serum N-glycosylation profiles, using mass spectrometry, were monitored at weekly intervals, for 7 weeks, to evaluate baseline levels and normal fluctuations. The individuals were then monitored for a further 7 weeks, during which time increasing doses of AA were ingested (1.3–5.2 g/day). Results: No adverse events were encountered. AA supplementation resulted in distinct changes in the relative intensities of seven biantennary N-glycans (P |
| Databáze: | OpenAIRE |
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