| Autor: |
Nuño-Cabanes, Carme, García-Molinero, Varinia, Martín-Expósito, Manuel, María-Eugenia Gas, Oliete-Calvo, Paula, Encar García-Oliver, Iglesia-Vayá, María De La, Rodríguez-Navarro, Susana |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.13161453.v1 |
| Popis: |
Additional file 2: Figure S1. Sgf73 peptides detected by LC MS/MS and MASCOT in Sus1-TAP precipitates from different SAGA mutants. The middle region of Sgf73, which was recently found to contact SAGA-CORE subunits [17] is highlighted in blue. Some of the Sgf73 peptides found by LC MS/MS are highlighted in red. Figure S2. Levels of Ubp8 protein tagged with GFP in WT and spt7Δ mutant cells were analysed in three different experiments (1, 2 and 3) with two replicates of Ubp8-GFPspt7Δ in each (a and b) by western blotting of whole-cell extracts using an anti-GFP antibody. Levels of Pgk1 protein were also monitored and used as the loading control. Cropped blots are shown for clarity. Full-length blots are presented in Figure S6. Figure S3. Full-length blot that was cropped for Fig. 1e. Figure S4. Full-length blot that was cropped for Fig. 4a. Figure S5. Full-length blot that was cropped for Fig. 4c (upper and lower panels). Figure S6. Full-length blots that were cropped for Figure S2 (upper panel). Figure S7. Different full microscope images used in Figs. 2 and 3. Figure S8. Detailed GFP Fluorescence microscopic quantification using IPython notebooks. To determine significant values between different experimental groups, the mean data were compared using one-way analysis of variance (ANOVA). Tukey's multiple comparisons test was also used. Values of *p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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