Microbial carbon metabolism associated with electrogenic sulphur oxidation in coastal sediments
| Autor: | Vasquez-Cardenas, Diana, van de Vossenberg, Jack, Polerecky, Lubos, Malkin, Sairah Y., Schauer, Regina, Hidalgo-Martinez, Silvia, Confurius, Veronique, Middelburg, Jack J., Meysman, Filip J R, Boschker, Henricus T S, Geochemistry, General geochemistry |
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| Přispěvatelé: | Earth System Sciences, Chemistry, Geochemistry, General geochemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Deltaproteobacteria
Geologic Sediments DNA Complementary Evolution Microorganism Microbiology Mass Spectrometry Carbon Cycle Electron Transport 03 medical and health sciences Microbial ecology Behavior and Systematics Gammaproteobacteria 14. Life underwater Autotroph Electrodes In Situ Hybridization Fluorescence Ecology Evolution Behavior and Systematics 030304 developmental biology Carbon Isotopes 0303 health sciences Bacteria biology Ecology 030306 microbiology Fatty Acids Carbon fixation biology.organism_classification Carbon Oxygen Biochemistry Original Article Oxidation-Reduction Biomarkers Sulfur Environmental Monitoring Desulfobulbaceae |
| Zdroj: | The ISME Journal, 9(9), 1966. Nature Publishing Group The ISME Journal Vasquez-Cardenas, D, van de Vossenberg, J, Polerecky, L, Malkin, S Y, Schauer, R, Hidalgo-Martinez, S, Confurius, V, Middelburg, J J, Meysman, F JR & Boschker, H TS 2015, ' Microbial carbon metabolism associated with electrogenic sulphur oxidation in coastal sediments ', I S M E Journal, vol. 9, no. 9, pp. 1966-78 . https://doi.org/10.1038/ismej.2015.10 |
| ISSN: | 1751-7362 |
| Popis: | Recently, a novel electrogenic type of sulphur oxidation was documented in marine sediments, whereby filamentous cable bacteria (Desulfobulbaceae) are mediating electron transport over cm-scale distances. These cable bacteria are capable of developing an extensive network within days, implying a highly efficient carbon acquisition strategy. Presently, the carbon metabolism of cable bacteria is unknown, and hence we adopted a multidisciplinary approach to study the carbon substrate utilization of both cable bacteria and associated microbial community in sediment incubations. Fluorescence in situ hybridization showed rapid downward growth of cable bacteria, concomitant with high rates of electrogenic sulphur oxidation, as quantified by microelectrode profiling. We studied heterotrophy and autotrophy by following (13)C-propionate and -bicarbonate incorporation into bacterial fatty acids. This biomarker analysis showed that propionate uptake was limited to fatty acid signatures typical for the genus Desulfobulbus. The nanoscale secondary ion mass spectrometry analysis confirmed heterotrophic rather than autotrophic growth of cable bacteria. Still, high bicarbonate uptake was observed in concert with the development of cable bacteria. Clone libraries of 16S complementary DNA showed numerous sequences associated to chemoautotrophic sulphur-oxidizing Epsilon- and Gammaproteobacteria, whereas (13)C-bicarbonate biomarker labelling suggested that these sulphur-oxidizing bacteria were active far below the oxygen penetration. A targeted manipulation experiment demonstrated that chemoautotrophic carbon fixation was tightly linked to the heterotrophic activity of the cable bacteria down to cm depth. Overall, the results suggest that electrogenic sulphur oxidation is performed by a microbial consortium, consisting of chemoorganotrophic cable bacteria and chemolithoautotrophic Epsilon- and Gammaproteobacteria. The metabolic linkage between these two groups is presently unknown and needs further study. |
| Databáze: | OpenAIRE |
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