Investigation of the Potential Effects of Alpha-Lipoic Acid on Human Degenerated Intervertebral Disc Tissue Primary Cell Cultures
| Autor: | Hanefi Özbek, Duygu Yasar Sirin, Irfan Aydin, Numan Karaarslan |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Pathology
medicine.medical_specialty Thioctic Acid business.industry Alpha-Lipoic Acid Primary Cell Culture Intervertebral Disc Degeneration Cartilage Oligomeric Matrix Protein Extracellular Matrix Chondroadherin Gene Degenerated intervertebral disc Cell culture Medicine Humans Surgery Neurology (clinical) Interleukin-1 Beta business Matrix Metalloproteinase Intervertebral Disc |
| Zdroj: | Turkish neurosurgery. 32(3) |
| ISSN: | 1019-5149 |
| Popis: | AIM: To investigate the supplementation of alpha-lipoic acid (ALA) at the molecular level to determine its effect on primary cell cultures prepared from human intervertebral disc (IVD) tissue in an in vitro environment. MATERIAL and METHODS: Human primary cell cultures were prepared from IVD tissue resected during surgery. While cell cultures without ALA supplementation formed the control group, those with ALA supplementation formed the study group. All cell groups were stained using acridine orange/propidium iodide (AO/PI), and the incidence of apoptotic cell death was determined under a fluorescent microscope. Cell surface morphology and extracellular matrix (ECM) structures were evaluated under an invert light microscope. Simultaneously, cell proliferation was evaluated by MTT–ELISA analysis, and the expressions of chondroadherin (CHAD), cartilage oligomeric protein (COMP), interleukin-1 beta (IL-1β), and matrix metalloproteinase (MMP)-7 and-19, which are genes associated with ECM regulation, were tested using qRT–PCR. The data obtained were evaluated statistically using Tukey’s honestly significant difference (HSD) test after analysis of variance (ANOVA) was performed. The alpha significance value was accepted as |
| Databáze: | OpenAIRE |
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