PYRIDINE-NUCLEOTIDE COENZYMES IN BULL AND RABBIT SPERMATOZOA
| Autor: | G. D'alessio, E. Leone, M. Bistocchi |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Male
endocrine system Embryology Nicotinamide adenine dinucleotide Cofactor chemistry.chemical_compound Endocrinology Semen Animals Incubation reproductive and urinary physiology Epididymis chemistry.chemical_classification biology urogenital system Lability Obstetrics and Gynecology Cell Biology NAD Spermatozoa Enzyme Reproductive Medicine Biochemistry chemistry biology.protein Cattle Rabbits NAD+ kinase Pyridine nucleotide NADP Nicotinamide adenine dinucleotide phosphate |
| Zdroj: | Reproduction. 16:223-231 |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | The content of the two pyridine-nucleotide coenzymes, namely nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP), has been determined in bull and rabbit spermatozoa, using the enzymic-fluorimetric procedure of Bassham, Birt, Hems & Loening (1959). It has been found necessary to wash spermatozoa thoroughly before extraction, so as to remove all traces ofseminal plasma enzymes which otherwise would have degraded the coenzymes in the course of the analytical procedure. In epididymal spermatozoa a markedly higher content of pyridine-nucleotide coenzymes has been found, compared to that in ejaculated spermatozoa. Incubation experiments distinctly indicated a lability of the coenzymes in both types of spermatozoa. It has also been shown that a very short (1 min) incubation of epididymal spermatozoa with seminal plasma causes a definite decrease in the coenzyme content. |
| Databáze: | OpenAIRE |
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