Low-fluence rate, long duration photodynamic therapy in glioma mouse model using organic light emitting diode (OLED)
Autor: | Yi Jang Lee, Po Hsiung Chen, Yei San Hsieh, Han Wen Guo, Hsing Wen Wang, Meng Huan Ho, Wan Ting Huang, Shih Hwa Chiou, Liang Ting Lin, Chen-Yuan Dong |
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Rok vydání: | 2014 |
Předmět: |
medicine.medical_specialty
Time Factors medicine.medical_treatment Biophysics Urology Mice Nude Protoporphyrins Photodynamic therapy Pilot Projects Dermatology Mice Nude mouse Glioma medicine Animals Humans Pharmacology (medical) Photosensitizer Short duration Photosensitizing Agents Low fluence biology business.industry Aminolevulinic Acid Tumor Oxygenation biology.organism_classification medicine.disease Survival Analysis Surgery Radiation therapy Disease Models Animal Oncology Photochemotherapy business Glioblastoma |
Zdroj: | Photodiagnosis and photodynamic therapy. 12(3) |
ISSN: | 1873-1597 |
Popis: | Summary Background The treatment of gliomas poses significant clinical challenges due to resistance to chemo and radiation therapy, and treatment side effects. Metronomic photodynamic therapy (mPDT), which involves long treatment time with low fluence rate and multiple or continuous photosensitizer administrations, has potential in treating gliomas without threatening the quality of life and has been demonstrated in rats and rabbits. mPDT in small animals such as mouse is not yet shown due to lack of lightweight illumination device for long periods of time. Methods We presented low fluence rate (3 mW/cm2) and long duration (3.7 h) PDT treatment in a nude mouse model of human glioblastoma by using organic light emitting diode (OLED) with single dose of 5-aminolevulinic acid (ALA) administration as photosensitizer. Tumor volume was measured using bioluminescent imaging and the animal survival time was recorded. Additionally, we have performed limited PDT dosimetric measurements of PpIX fluorescence, tumor oxygenation and hemoglobin concentration in 3 PDT mice. Results For animals with similar pre- and immediate post-light tumor volume, the averaged total survival time of PDT mice is 40.5 ± 9.2 days that are significantly longer than the control mice (26.0 ± 2.0 days). The post-light survival time of PDT mice is 14.3 ± 5.9 days that are marginally longer than the control group (8.0 ± 0.0 days). In the dosimetric measurement, good maintenance of PpIX fluorescence in one PDT mouse has relatively improved survival time, compared with the other two PDT mice (i.e., 24 days versus 16 and 17 days). Conclusions This pilot study demonstrated the feasibility of low-fluence rate and long treatment time of ALA-PDT using OLED without anesthetization of animals. The response of PDT treated animals with similar pre- and post-light tumor volume is encouraging to show a longer survival time than the controls. The dosimetric indices such as photosensitizer fluorescence and tissue oxygenation would help understand the possible treatment barriers for further improvement of treatment plans. |
Databáze: | OpenAIRE |
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