Purification and Characterization of Plant Dynamin from Tobacco BY-2 Cells
| Autor: | Takashi Hashimoto, Takahiro Hamada, Teruo Shimmen, Maki Yao, Seiji Sonobe, Hisako Igarashi |
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| Rok vydání: | 2006 |
| Předmět: |
Dynamins
Tobacco BY-2 cells biology Arabidopsis Proteins Physiology Nicotiana tabacum macromolecular substances Cell Biology Plant Science General Medicine biology.organism_classification Molecular biology In vitro law.invention Microtubule law Tobacco Arabidopsis thaliana Electron microscope Microtubule-Associated Proteins Cells Cultured Actin Dynamin |
| Zdroj: | Plant and Cell Physiology. 47:1175-1181 |
| ISSN: | 1471-9053 0032-0781 |
| Popis: | We purified an 84 kDa polypeptide from the MAP (microtubule-associated protein) fraction of tobacco BY-2 cultured cells. LC/MS/MS (liquid chromatography-tandem mass spectrometry) analysis revealed that this polypeptide is a tobacco homolog of AtDRP3 (Arabidopsis thaliana dynamin-related protein 3). Electron microscopy revealed that NtDRP3 (Nicotiana tabacum dynamin-related protein 3) assembles to form a filamentous structure. When GDP was added to the NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind to and bundle both microtubules and actin filaments in vitro. |
| Databáze: | OpenAIRE |
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