Nucleocytoplasmic interactions during ontogenesis in individuals obtained by intra- and interspecific nuclear transplantation in the genus Pleurodeles (Urodele Amphibian)
| Autor: | Claude-Louis Gallien, Francoise Guillet, Christian Aimar |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Graft Rejection
Pleurodeles Amphibian Cytoplasm Nuclear Transfer Techniques Genotype Ontogeny Extrachromosomal Inheritance Pleurodeles poireti Malate dehydrogenase Amphibians Malate Dehydrogenase biology.animal Botany Animals Transplantation Homologous Molecular Biology Ovum L-Lactate Dehydrogenase biology Embryo Skin Transplantation Cell Biology biology.organism_classification Blastula Cell biology Phenotype Hybridization Genetic Female Developmental Biology |
| Zdroj: | Developmental Biology. 33:154-170 |
| ISSN: | 0012-1606 |
| Popis: | Nuclei derived from a single blastula of Pleurodeles waltlii or Pleurodeles poireti are transplanted to the cytoplasm of eggs of both species ( P. waltlii and P. poireti ). Animals with identical genomes are obtained, some resulting from an intraspecific nucleocytoplasmic association, the others from an interspecific association (nucleocytoplasmic hybrids). A marked lethality results from the heterospecific association. Morphological and physiological effects, concomitant to initial egg conditioning, occur during the first stages of development. The study of lactic dehydrogenase (LDH) and malic dehydrogenase (MDH) zymograms reveals the genomic activity in the embryo from stage 28 (heart beat). A regionalization of this activity is evident; the nuclear activity can be shown in the ectomesodermic tissue sooner than in the endodermal cells. From stage 38 (feeding), the functional substitution of transplanted nucleus for effects of initial cytoplasmic conditioning appears to be total. No cytoplasmic heredity can be shown: the genetic effect of the transplanted nucleus is dominant. This is corroborated by the results of cutaneous cross-grafts between adult allocytoplasmic twins. Those animals present strictly identical immune reactions; their antigenic constitution seems to be independent from the specific origin of the egg cytoplasm. |
| Databáze: | OpenAIRE |
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