Characterization and genetic analyses of a carbazole-degrading gram-positive marine isolate, Janibacter sp. strain OC11
| Autor: | Shintaro Oba, Toshio Omori, Hiroyuki Fuse, Rintaro Maeda, Toshihiro Suzuki |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Aquatic Organisms Transcription Genetic Operon Molecular Sequence Data Carbazoles Artificial seawater Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Bacterial Proteins Actinomycetales Gene cluster Amino Acid Sequence Molecular Biology Gene Southern blot Genetics biology Organic Chemistry Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Molecular biology Open reading frame human activities Bacteria Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 78:1094-1101 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2014.917260 |
| Popis: | Strain OC11 was isolated from seawater sampled at the coast of Chiba, Japan, in artificial seawater medium with carbazole (CAR) as the sole carbon source. Its 16S ribosomal RNA gene sequence suggested that strain OC11 belongs to the genus Janibacter. The CAR-degradation genes (car genes) of strain OC11 were PCR amplified, using degenerate primers designed based on the car gene sequences of other CAR-degrading bacteria. Complete nucleotide sequences encoding six complete open reading frames were determined, and the first known ferredoxin reductase gene (carAd) was found from a CAR-degrading bacterium isolated from the marine environment. An experiment using a mutant strain suggested that the car genes of strain OC11 are functional in CAR degradation. Southern hybridization indicated that strain OC11 had one car gene cluster in vivo. RT-PCR revealed that transcription of carOC11 constitutes an operon. |
| Databáze: | OpenAIRE |
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