Molecular Cloning of a Novel Transmembrane Protein MOLT Expressed by Mature Oligodendrocytes
| Autor: | Kouichi Itoh, Toshitaka Oohashi, Tomoko Yonezawa, Fumio Shiraga, Kiyotaka Yamamoto, Katsuhiko Ono, Hiroshi Ohtsuki, Yoshifumi Ninomiya, Hiroyuki Nomoto |
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| Rok vydání: | 2003 |
| Předmět: |
Signal peptide
DNA Complementary Molecular Sequence Data Nerve Tissue Proteins Protein Sorting Signals Biology Biochemistry Mice Complementary DNA Chlorocebus aethiops medicine Animals Humans Cell Lineage Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Peptide sequence Cells Cultured Mice Inbred BALB C Messenger RNA Sequence Homology Amino Acid fungi Brain Membrane Proteins Cell Differentiation General Medicine Molecular biology Transmembrane protein Oligodendrocyte Rats Oligodendroglia Transmembrane domain medicine.anatomical_structure Cell culture COS Cells Endothelium Vascular |
| Zdroj: | Journal of Biochemistry. 134:231-238 |
| ISSN: | 0021-924X |
| DOI: | 10.1093/jb/mvg135 |
| Popis: | A novel oligodendrocyte (OL)-specific cDNA was isolated from brain capillary endothelial cells and characterized. The cDNA encodes a protein of 1099 amino acids that contains a signal peptide and a transmembrane domain. The protein was expressed in mature OLs in vivo and in vitro cell cultures and was thus designated as mature OL transmembrane protein (MOLT). RT-PCR analysis showed that MOLT mRNA was expressed in brain, lung, pancreas, and testis. A polyclonal antibody raised against a part of the mouse MOLT reacted specifically with multipolar OLs possessing radially oriented processes that penetrated into the gray matter. More cells were detected in the white matter, and these had longitudinally oriented processes. In a rat OL lineage culture system, oligodendrocyte precursor cells did not initially produce MOLT mRNA and protein, but when they begun to differentiate into mature OLs, they started expressing MOLT. Consequently, MOLT may function as OLs become mature and may serve as a cell-surface marker for OL differentiation. |
| Databáze: | OpenAIRE |
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