Predictive value of multidrug resistance proteins and cellular drug resistance in childhood relapsed acute lymphoblastic leukemia

Autor: Anna Balcerska, Mariusz Wysocki, Elzbieta Stanczak, Wanda Badowska, Michał Matysiak, Edyta Juraszewska, Dorota Olszewska-Słonina, Sylwia Kołtan, Walentyna Balwierz, Beata Kolodziej, Marta Kuzmicz, Andrzej Kołtan, Monika Pogorzała, Krzysztof Czyżewski, Jolanta Stefaniak, Danuta Sońta-Jakimczyk, Jan Styczyński, Benigna Konatkowska, Lucyna Maciejka-Kapuscinska, Andrzej Kurylak, Maryna Krawczuk-Rybak, Jerzy Kowalczyk, Malgorzata Kubicka, Lidia Gil, Igor Olejnik, Robert Dębski, Beata Rafinska, Tomasz Szczepański, Jacek Wachowiak, Maria Wieczorek, Iwona Malinowska
Rok vydání: 2007
Předmět:
Oncology
Adult
Male
medicine.medical_specialty
Cancer Research
Myeloid
Adolescent
ATP-binding cassette transporter
Antineoplastic Agents
Drug resistance
Immunophenotyping
Multidrug Resistance Protein 1
hemic and lymphatic diseases
Acute lymphocytic leukemia
Internal medicine
Medicine
Humans
ATP Binding Cassette Transporter
Subfamily B
Member 1
Child
Vault Ribonucleoprotein Particles
Hematology
business.industry
Gene Expression Regulation
Leukemic
Infant
Newborn
Infant
General Medicine
Precursor Cell Lymphoblastic Leukemia-Lymphoma
medicine.disease
Flow Cytometry
Prognosis
Drug Resistance
Multiple
Multiple drug resistance
Leukemia
medicine.anatomical_structure
Drug Resistance
Neoplasm
Child
Preschool
Immunology
Female
Multidrug Resistance-Associated Proteins
Neoplasm Recurrence
Local
business
Zdroj: Journal of Cancer Research and Clinical Oncology. 133:895-895
ISSN: 1432-1335
0171-5216
DOI: 10.1007/s00432-007-0299-5
Popis: Cellular resistance in childhood acute leukemias might be related to profile and function of multidrug resistance proteins and apoptosis regulating proteins. The aims of the study were: (1) analysis of expression of MRP1, PGP1, LRP, BCL-2 and p53 proteins; (2) correlation with ex vivo drug resistance, and (3) analysis of their prognostic impact on clinical outcome in childhood acute lymphoblastic (ALL) and acute myeloid (AML) leukemia. Total number of 787 children diagnosed for initial ALL (n = 527), relapsed ALL (n = 104), initial AML (n = 133) and relapsed AML (n = 23) were included into the study. Mean follow-up period was 3.5 years. Drug resistance for up to 30 anticancer agents was performed by the MTT assay. Expression of all proteins was tested by flow cytometry. Both initial AML and relapsed ALL samples showed higher drug resistance than initial ALL samples. No significant differences were found in drug resistance between initial and relapsed AML samples. The presence of multidrug resistance and apoptosis proteins had no impact on pDFS in iALL and iAML, however strong trend towards adverse prognostic impact of MRP1, PGP and LRP on pDFS in rALL was observed. The same trend was observed for each of analyzed co-expressions of tested multidrug resistance proteins. The phenomenon of cellular drug resistance in childhood acute leukemias is multifactorial and plays an important role in response to therapy. Expression of MRP1, PGP and LRP proteins, as well as their co-expression play possible role in childhood relapsed ALL.
Databáze: OpenAIRE
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