The Osteoinductive Effect of Controlled Bone Morphogenic Protein 2 Release Is Location Dependent
| Autor: | Diederik H.R. Kempen, Michael J. Yaszemski, Marianna A. Tryfonidou, Björn P. Meij, Lichun Lu, Jacqueline Alblas, Loek D. Loozen, Behdad Pouran, Wouter J.A. Dhert, Maurits Geert Laurent Olthof |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Scaffold oligo[(polyethylene glycol) fumarate] 0206 medical engineering Implantation Site Biomedical Engineering Bioengineering 02 engineering and technology Polyethylene glycol bone morphogenetic protein 2 Bone morphogenetic protein Bone tissue Bone morphogenetic protein 2 Biochemistry Bone and Bones Polyethylene Glycols Rats Sprague-Dawley Biomaterials 03 medical and health sciences chemistry.chemical_compound medicine Animals Humans application sites bone tissue engineering 030304 developmental biology 0303 health sciences Tissue Engineering Tissue Scaffolds Hydrogels Original Articles Phosphate 020601 biomedical engineering Rats medicine.anatomical_structure chemistry Collagen sponge Delayed-Action Preparations Biomedical engineering |
| Zdroj: | Tissue Engineering-Part A, 25(3-4), 193. Mary Ann Liebert Inc. |
| ISSN: | 1937-3341 |
| Popis: | The main challenge in bone morphogenic protein 2 (BMP-2)-based application lies in finding strategies that prolong its effective period as it has a short biological half-life. Several BMP-2 release profiles have shown to enhance bone formation at various application sites. However, it remains to be determined which BMP-2 release profile best augments bone formation and whether this effect is location dependent. Therefore, the aim of this study was to investigate the effect of BMP-2 release from oligo[(polyethylene glycol) fumarate] bis(2-(methacryloyloxy)ethyl) phosphate (OPF-BP) composites on the osteoinductive efficacy at ectopic versus orthotopic application. By varying the BMP-2 loading method, three different OPF-BP composites were created with varied release profiles. The composites were compared with unloaded OPF-BP as negative control, and to the clinically used Infuse(®) absorbable collagen sponge (ACS) as positive control. Bone formation was assessed by microcomputed tomography after 9 weeks of subcutaneous implantation and 3, 6, and 9 weeks of orthotopic implantation in rats (n = 48). Whereas a BMP-2 burst release of >49% generated significantly more bone compared with sustained release (burst release |
| Databáze: | OpenAIRE |
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