Correlation of serostatus and viraemia levels among Indian dengue patients at the time of first diagnosis
| Autor: | Divya Tiraki, Harshad P. Patil, Ruta Kulkarni, Vidya A. Arankalle, Akhilesh Chandra Mishra, Shubham Shrivastava |
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| Rok vydání: | 2020 |
| Předmět: |
India
Enzyme-Linked Immunosorbent Assay Viral Nonstructural Proteins Dengue virus Antibodies Viral medicine.disease_cause Sensitivity and Specificity Virus Dengue fever Serology Dengue Chlorocebus aethiops medicine Animals Humans Viremia Vero Cells biology business.industry Infectious dose Public Health Environmental and Occupational Health virus diseases General Medicine Dengue Virus medicine.disease Virology Titer Infectious Diseases Immunoglobulin M biology.protein Parasitology business Serostatus |
| Zdroj: | Transactions of The Royal Society of Tropical Medicine and Hygiene. 114:513-520 |
| ISSN: | 1878-3503 0035-9203 |
| DOI: | 10.1093/trstmh/traa027 |
| Popis: | BackgroundDengue is a public health problem worldwide. Therapeutic monoclonal antibodies (MAbs) against dengue virus (DENV) are likely to be available soon. In view of the feasibility issues pertaining to pretreatment viraemia quantitation for therapy decisions, we conducted this study for investigation of a correlation between patient serostatus (NS1/immunoglobulin M [IgM]/IgG) and viraemia levels among Indian dengue patients at the time of first diagnosis.MethodsThe study included 297 serum samples from dengue patients in Pune, India. The samples were tested for NS1, IgM and IgG (capture enzyme-linked immunosorbent assay [ELISA] for identifying secondary dengue) using Panbio ELISAs. Quantitation of viraemia was conducted using an NS1 ELISA-based 50% tissue culture infectious dose (TCID50) test in Vero cells.ResultsViraemia was detectable only among NS1-positive patients (n = 229, range 0.5–8.3 logTCID50/ml) with a mean titre of 1.9 logTCID50/ml. Among the NS1-positive patients, DENV titres were higher in IgM-negative than IgM-positive patients (p < 0.0001) and in primary (IgG < 18 Panbio units) versus secondary (IgG > 22 Panbio units) dengue patients (p = 0.002). Virus titres were higher during the first 3 days of illness and decreased later (p = 0.005).ConclusionsThe study provides a range of infectious DENV titres in relation to serologic status among dengue patients in India. The data suggest the possibility of using serological markers (NS1/IgM) as a basis for treatment decisions. |
| Databáze: | OpenAIRE |
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