Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish, Carassius auratus
| Autor: | Hossam F. Nassar, Koji Yachiguchi, Yuji Oshima, Hiroyuki Mishima, Makoto Urata, Masayuki Sato, Yoshiaki Tabuchi, Kazuichi Hayakawa, Akira Toriba, Ning Tang, Samah M. Bassem, Fagr Kh. Abdel-Gawad, Chun Sang Hong, Nobuo Suzuki, Toshio Sekiguchi, Y. Shimasaki, Atsuhiko Hattori, Masato Endo, Fumiya Makino |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Genetic Markers
0301 basic medicine musculoskeletal diseases polycyclic aromatic hydrocarbons 010501 environmental sciences 01 natural sciences 03 medical and health sciences Carassius auratus Animals Bioassay Seawater RNA Messenger fish scales goldfish 0105 earth and related environmental sciences nitro-polycyclic aromatic hydrocarbons biology Acid phosphatase osteoblasts Anatomy Enzyme assay Dilution 030104 developmental biology osteoclasts Gene Expression Regulation bioassay Environmental chemistry biology.protein Osteocalcin Alkaline phosphatase Animal Science and Zoology bone metabolism Integumentary System Water Pollutants Chemical |
| Zdroj: | Zoological Science. 33(4):407-413 |
| ISSN: | 0289-0003 |
| Popis: | We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from two sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We were able to detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added to culture medium at dilution rates of 50, 100, and 500, and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, but TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-κB ligand) decreased significantly, as did the ALP enzyme activity. In fact, ALP activity decreased on treatment with PAHs and NPAHs. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influences bone metabolism in teleosts. © 2016 Zoological Society of Japan. Embargo Period 12 months |
| Databáze: | OpenAIRE |
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