Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles
| Autor: | Mariella Ravazzola, Alain Perrelet, M. J. Storch, Lelio Orci, Richard G.W. Anderson, Jean-Dominique Vassalli |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Peptide Hydrolases/metabolism
medicine.medical_treatment Immunocytochemistry Coated vesicle Insulin/immunology/ metabolism Cytoplasmic Granules Clathrin General Biochemistry Genetics and Molecular Biology Proinsulin/ metabolism symbols.namesake medicine Insulin Cytoplasmic Granules/ physiology ddc:576.5 Clathrin/physiology Proinsulin Antibodies Monoclonal/diagnostic use biology Antibodies Monoclonal Hydrogen-Ion Concentration Golgi apparatus Secretory Vesicle Cell Compartmentation Microscopy Electron Secretory protein Gold/diagnostic use Biochemistry biology.protein symbols Gold Protein Processing Post-Translational Peptide Hydrolases |
| Zdroj: | Cell, Vol. 49, No 6 (1987) pp. 865-868 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(87)90624-6 |
| Popis: | The direct identification of the intracellular site where proinsulin is proteolytically processed into insulin has been achieved by immunocytochemistry using an insulin-specific monoclonal antibody. Insulin immunoreactivity is absent from the Golgi stack of pancreatic B-cells and first becomes detectable in clathrin-coated secretory vesicles released from the trans Golgi pole. Clathrin-coated secretory vesicles transform into mature noncoated secretory granules which contain the highest concentration of insulin immunoreactive sites. Maturation of clathrin-coated secretory vesicles is accompanied by a progressive acidification of the vesicular milieu, as evidenced by a cytochemical probe that accumulates in acidic compartments whereupon it can be revealed by immunocytochemistry. Thus packaging of the prohormone in secretory vesicles, and acidification of this compartment, are critical steps in the proper proteolytic maturation of insulin. |
| Databáze: | OpenAIRE |
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