Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients
| Autor: | Zsolt Tulassay, Bernadett Toth, Lajos Floro, Béla Molnár, Ferenc Sipos, Lydia Sreter |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
CK-20 RT-PCR
Adult Male Pathology medicine.medical_specialty Colorectal cancer microscopic counting Clinical Biochemistry Cell colorectal cancer Buffy coat Uicc stage Gastroenterology Cytokeratin Circulating tumor cell Internal medicine Genetics medicine Biomarkers Tumor Humans RNA Messenger Molecular Biology neoplasms Aged lcsh:R5-920 business.industry Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) Circulating tumor cells General Medicine Middle Aged medicine.disease Neoplastic Cells Circulating Peripheral blood digestive system diseases medicine.anatomical_structure Cancer cell Disease Progression Female Other progression lcsh:Medicine (General) business Colorectal Neoplasms |
| Zdroj: | Disease markers Disease Markers, Vol 24, Iss 3, Pp 141-150 (2008) |
| ISSN: | 1875-8630 0278-0240 |
| Popis: | Aims:Cytokeratin(CK) based real-time RT-PCR assays (QRT-PCR) are now available for peripheral blood circulating tumor cell (CTC) evaluations in colorectal cancer(CRC) patients. Results are non-existent for the application of these techniques to the determination of progression and therapy response in Dukes stage D CRC patients.Patients and methods:Each month 30 ml peripheral blood of 30 Dukes D patients (17 with progression) were drawn. CEA, CA19-9, CA72-A and TPA-M determinations were made. CK20, thymidilate synthase(TS) QRT-PCR was performed, as well. Buffy coat was used for immunmagnetic cancer cell isolation and CTC counting. Correlation between elevated CTC and macroscopic progression within 3 months was determined by Chi2test.Results:Microscopic CTC single cell, doublet, cluster number were found in correlation with CK20 QRT-PCR results (p< 0.01). There was a significant increase in microscopic CTC number, CK20 and decrease in TS QRT-PCR levels (p< 0.05) in the peripheral blood of the non-responder as compared to responder patients. Elevation of the CTC was in significant correlation with macroscopic progression of the disease in 3 months (p< 0.01).Conclusions:CTC number reflects the chemotherapeutic sensitivity of CRC patients. Elevation of circulating tumor cell number in peripheral blood is in correlation with macroscopic progression. |
| Databáze: | OpenAIRE |
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