Bacteriophage cooperation suppresses CRISPR-Cas3 and Cas9 immunity
| Autor: | Joseph Bondy-Denomy, Adair L. Borges, Beatriz A. Osuna, Blake Wiedenheft, MaryClare F. Rollins, Jenny Y. Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
anti-CRISPR
0301 basic medicine viruses Cell host-pathogen interaction Genome Medical and Health Sciences Bacteriophage 0302 clinical medicine bacteriophage CRISPR Bacteriophages skin and connective tissue diseases Genetics 0303 health sciences education.field_of_study biology CRISPR-Cas immunity Biological Sciences medicine.anatomical_structure Infectious Diseases Pseudomonas aeruginosa Host-Pathogen Interactions CRISPR-Cas9 Infection musculoskeletal diseases Evolution Host–pathogen interaction Population cooperation virus General Biochemistry Genetics and Molecular Biology Virus Article Evolution Molecular Viral Proteins 03 medical and health sciences Immune system Immunity medicine education 030304 developmental biology 030306 microbiology Cas9 Inflammatory and immune system Molecular biology.organism_classification Virology Emerging Infectious Diseases 030104 developmental biology altruism CRISPR-Cas Systems 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Cell, vol 174, iss 4 |
| Popis: | SUMMARY>Bacteria utilize CRISPR-Cas adaptive immune systems for protection from bacteriophages (phages), and some phages produce anti-CRISPR (Acr) proteins that inhibit immune function. Despite thorough mechanistic and structural information for some Acr proteins, how they are deployed and utilized by a phage during infection is unknown. Here, we show that Acr production does not guarantee phage replication, but instead, infections fail when phage population numbers fall below a critical threshold. Failing infections can be rescued by related phages that act as Acr donors, demonstrating that infections succeed if a sufficient Acr dose is contributed to a single cell by multiple phage genomes. The production of Acr proteins by phage genomes that fail to replicate leave the cell immunosuppressed, which predisposes the cell for successful infection by other phages in the population. This “cooperative” phage mechanism for CRISPR-Cas inhibition demonstrates inter-virus cooperation that may also manifest in other host-parasite interactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|